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projects: chromatin enzymes
projects: chromaint factors

projects: DNA ladders
projects: polycistronic expression
picture & movie gallery
gallery: amino acids
gallery: secondary structure
gallery: selected proteins
gallery: protein/DNA complexes

background reading
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positions

 
Made on a Mac
 

 

DNA ladders also known as DNA molecular weight markers are among the most commonly used reagents in molecular biology research. DNA ladders function as molecular rulers, allowing one to determine the size of DNA fragments.

We have constructed two plasmids that produce 1 kb or 100 bp DNA ladders when digested with the EcoRV or the PstI restriction enzyme, respectively. Unlike many currently available DNA ladders, the 100 bp ladders migrate appropriately on both agarose and polyacrylamide gels. 100 ml of E. coli cultures for each of the two plasmids is sufficient to prepare 100 bp or 1 kb DNA ladders for 1 000 lanes. The plasmids allow the production of DNA ladders at less than a penny a lane, a fraction of the cost of using commercial DNA ladders.

The plasmids to produce the Penn State DNA ladders are available without licensing restrictions to nonprofit academic users through the Addgene plasmid repository:

pPSU1 plasmid at Addgene

pPSU2 plasmid at Addgene

Instructions for preparing the Penn State DNA ladders from the plasmids.

Penn State News article about the project.

This project was driven by undergraduates in the laboratory.

We are grateful for funding support from:
Penn State (Eberly College of Science Undergraduate Research Support) and
NIH National Institute of General Medical Sciences.

References:

Henrici, R.C., Pecen, T.J., Johnston, J.L and Tan, S. (2017) The pPSU plasmids for generating DNA molecular weight markers, Scientific Reports, 7:2438.

 


video introduction | projects: | chromatin enzymes | chromatin factors | DNA ladders | polycistronic expression | picture & movie gallery: | amino acids | secondary structure | selected proteins | protein/DNA complexes |
background reading | reprints | positions |
lab pictures |


This page is maintained by Song Tan: sxt30@psu.edu (814-865-3355)
Department of Biochemistry & Molecular Biology, 108 Althouse Lab, University Park, PA 16802

This page was last updated on September 14, 2017.

Penn State BMB Eberly College of Science