M.C.Demirel LAB-RESEARCH FACILITIES

 

The laboratories of Prof. Demirel’s are on the 1st floor of the Earth and Engineering Science Building. (Room 107 and 114A)

Current permanent equipments located in include:

Experimental:

  • Atomic Force Microscope, Nanoscope E

  • Polymer Vapor Deposition System (custom-made)

  • The Nicolet FT-IR/ATR (Thermo)

  • Spectrometer-Nanodrop

  • UV/Vis Spectrophometer 

  • 1 Olympus inverted microscopes

  • UV apparatus

  • Shaking water bath

  • Beckman refrigerated centrifuge

  • 1 microcentrifuges

  • 1 environmetal incubator/shaker

  • 2 refrigerators, -80oC and -20 oC freezers

  • SpeedVac vacuum dryer

  • Microwave, Thermoline heat bloks

  • 1 dry air incubator,

  • Perkin-Elmer DNA thermocycler-PCR

  • Mettler analytical balance

  • Drying ovens

  • pH meter

  • Autoclave

  • Nanopure Water system

  • 1 tissue culture CO2 incubators

  • 1 laminar floor hood

  • 1 chemical hood

  • Electroporator

  • Protein and DNA Gel electrophoresis

  • Rotary Evaporator (Rotovap)

  • Liquid nitrogen storage

 


AFM Nanoscope


Polymer Synthesis and Protein Expression Lab


Cell Culture Lab


 

Computational:

We have two high performance Pentium clusters:

1. A shared cluster (128 nodes) located at the Computer Buildiing  http://gears.aset.psu.edu/hpc/systems/lionxm/

2 Bionanomaterials group (26 nodes) cluster located at the Earth and Engineering Science Bldg. (see picture)

 



Computer Cluster

Core Facilities:

We frequently use the following shared core facilities at Penn State 

The Materials Characterization Lab (MCL) is a fully staffed analytical laboratory, which serves the materials community at Penn State offering a range of state-of-the-art analytical techniques and support services. MCL offers complete sample preparation, analysis, interpretation, report writing and consultation on over 20 different tools. MCL is part of the Materials Research Institute. For a complete list and description of the services we offer see below. Services offered include: Microscopy (STEM/TEM, SEM, FE-SEM, ESEM, AFM, OIM, EDS, FIB), Surface and Thin Film (XPS/ESCA, FE-Auger, EPMA), Structural Analysis (XRD, FTIR, Raman, UV-Vis, XRR, SAXS), Chemical Analysis (ICP, ICP-MS, IC, UV-Vis), Physical Property Determination (dielectric properties, powder characterization, thermal analysis DTA/TGA/DSC)  http://www.mri.psu.edu/mcl/

Penn State Nanofabrication Facility (www.nanofab.psu.edu) has the following equipments: Leo 440 scanning electron microscope (LaB6), Leo 1530 FESEM, Optical microscopy including Leitz Ergolux 200 w/ BF, DF, DIC, Tencor Instruments Alpha-Step 500, 200, and 100 profilometers, Bio-Rad FTIR, DLTS, and FTPL materials characterization systems, Micromanipulator Probe station,  HP4284A Precision LCR Meter and HP4291B RF impedance/material analyzer, Nanometrics Nanospec and Jandel 4 point probe, Dry Etching/Plasma Treatment (Applied Materials Precision 5000 MERIE, Applied Materials DPS ICP RIE tool, Plasma Therm RIE 720, Tepla America (formerly Metroline) M4L RIE, Annealing (AET RX-8 rapid thermal processor, several conventional furnaces and ovens, Metallization (Kurt Lesker e-gun and thermal evaporator, Varian 3125 e-gun and thermal evaporator, 2 Edwards thermal evaporators for Al and novel materials, Coupled SPM/SEM, Coupled AFM/Fluorescence microscope, Nanoimprinter and substrate bonder. http://www.nanofab.psu.edu/

Center for Quantitative Cell Analysis(CQCA) The CQCA is one of the Shared Technology Facilities of the Huck Institute of the Life Sciences. The mission of the CQCA is to provide the Penn State research community with state-of-the-art instruments and expertise in analytical flow and image cytometry. The Olympus Fluoview 300 Confocal Laser Scanning Microscope includes three single-line lasers with individual shutters that are software controlled for sequential acquisition. DIC is also supported on this system. Confocal LSM improves the resolution of conventional fluorescence images by recording fluorescence or reflected light generated when a laser is focused at set focal planes in cells and tissues, rejecting all other light coming from planes above or below the one of interest. The elimination of out-of-focus light improves resolution of submicron structures within fixed and live cells and tissues. A series of images can be recorded axially and either analyzed separately or be incorporated into a composite image. Cost = $30 per hour. The Olympus BX-60 is an epi-fluorescent upright microscope interfaced with a computer controlled focus drive and a Hamamatsu Orca-100 camera. AutoQuant deconvolution and Autovisualize-3D software use specific algorithms to deblur or remove out-of-focus haze. 3-D reconstruction software is used to produce a composite of deconvolved images. Cost = $15 per hour. http://hils.psu.edu/stf/home.html

DNA Microarray Facility: facility provides custom DNA microarray preparation, hybridization, and scanning services. Affymetrix GeneChip™ hybridization and scanning services are also available. In addition to microarray services, it provides bacterial colony picking and gridding services utilizing the Genetix QPixII™ robot which is housed in our facility. All services are provided only to Penn State researchers http://hils.psu.edu/stf/home.html

The Electron Microscopy Facility: The Facility serves as a training and service site for multi-user from the Penn State research community by providing expertise and instrumentation for the state-of-the-art practice on electron microscopy and light microscopy.   Some specific areas are conventional TEM and SEM, cryo-SEM, digitized imaging, microtomy, cryogenic sample preparation, high resolution imaging, elemental analysis, immunogold labeling, freeze-substitution, histology and immunohistochemistry. In addition, the Facility acts as an educational unit by providing students with hands-on practice of microscopy. The EM Facility is located on the ground floor of South Frear Building, in the central region of the University Park Campus. It is a 1800 sq ft suite of ten work areas for sample preparation, electron microscopy, optical light microscopy, ultramicrotomy, paraffin microtomy, digital imaging, darkroom procedures, and office space. Scanning Electron Microscope (SEM) Model: JEOL JSM 5400, Attachments: a C1500C cryotrans system and a PGT Prism light element detector This microscope allows users to perform conventional secondary electron imaging, backscatter electron imaging, X-ray microanalysis, and cryo-SEM. Transmission Electron Microscope (TEM), Model: JEOL JEM 1200 EXII, Attachments: Digital cameras: a video camera, a Gatan Bioscan 792 camera and a high resolution Tietz F224 camera; a PGT Prism light element detector , This microscope allows users to perform conventional TEM with thin-sectioned specimens,  x-ray microanalysis, and other transmission electron microscopy with specimens prepared using different methods. http://hils.psu.edu/stf/home.html

 

 

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