Questions/Topics to consider

A. Describe the function and regulation of the lac operon



B.  What are the characteristics of the following:









Homologous recombination




Restriction endonucleases


Virulent and temperate bacteriophages



C. Describe the key features of the different methods of DNA transfer in bacteria



1.      The region of a gene at which transcription ends is known as the:



a.      promoter


b.      operon


c.      operator


d.      terminator


2.      Enzymes that are expressed all the time (i.e. enzymes that are not regulated) are described as:

a.      inducible


b.      repressible


c.      constitutive


d.      Such enzymes do not exist.



3.      A set of genes that are coordinately expressed under the control of the same operator and promoter is known as a(n)

a.      regulon


b.      operon


c.      transcription unit


d.      none of the above



4.      The Polymerase Chain Reaction was discovered by:

a.      Griffiths


b.      Avery, MacLeod and McCarty


c.      Watson and Crick


d.      Mullis




5.      Which of the following processes involves the bacteriophage-mediated transfer of DNA between bacteria:

a.      transformation


b.      conjugation


c.      transposition


d.      transduction



6.      In which mixture of bacterial strains can conjugation occur?

a.      F+ + F+


b.      Hfr + Hfr


c.      Hfr + F+


d.      Hfr + F-