histoimage

Histological and Image Analysis Techniques

Weil Stain of Medial Parabrachial Nucleus Showing Marking Lesions

Lesion current: 3 uA for 3 Sec

Recording/lesion electrode: Glass coated tungsten with spherical tip

Tip profile: 3 uM X 12 uM X 3.5 uM (diameter at glass interface X length of exposed tungsten X tip diameter)

Weil stain by Kathleen Johnson: 50 uM section through "waist" area of PBN

Digital image by Sony DKC5000 camera - 24 bit RGB

Image processing by Picture Publisher, Photoshop, and Optimas 6.0

Two (maybe three) Lesions representing neuron locations of PBN cells sensitive to 0.1M NaCl

(best viewed at 16 bit or higher depth)

Enlargement of above image plus Compass filtering by Optimas Ver 6.0

(Notice the enhanced depth and cut edge of the blood vessel)

(Kathy: Please sharpen the blade!)