Publications.html

Nancy L. Scott§, Yu Xu, Gaozhong Shen, David A. Vuletich§, Christopher J. Falzone, Zhongkui Li, Marcus Ludwig, Matthew P. Pond, Matthew R. Preimesberger, Donald A. Bryant and Juliette T. J. Lecomte (2010) Functional and Structural Characterization of the 2/2 Hemoglobin from Synechococcus sp. PCC 7002. Biochemistry 49: 7000-7011.

Yuehui Zhu, Joel E. Graham, Marcus Ludwig, Wei Xiong, Richard M. Alvey, Gaozhong Shen and Donald A. Bryant (2010) Roles of xanthophyll carotenoids in protection against photoinhibition and oxidative stress in the cyanobacterium Synechococcus sp. strain PCC 7002. Achieves of Biochemistry and Biophysics. In press

Wendy M. Schluchter, Gaozhong Shen, Richard M. Alvey, Avijit Biswas, Nicolle A. Saunée, Shervonda R. Williams, Miller, C. A., and Donald A. Bryant (2010) Phycobiliprotein Biosynthesis in Cyanobacteria: Structure and Function of Enzymes Involved in Post-translational Modification. Recent Advances in Phototrophic Prokaryotes. Vol 675, Part3, pp211-228.
Vol 675, Part3, pp211-228.

van der Est, A., Chirico, S., Karyagina, I., Cohen, R., Shen, G., and Golbeck, J. H. (2010) Alteration of the axial Met ligand to electron acceptor A0 in Photosystem I: An investigation of electron transfer at different temperatures by multifrequency time-resolved and CW EPR. App. Magn. Res. 37: 103-121

Dong, C., Tang, A., Zhao, J., Mullineaux, C. W., Shen, G. and Bryant, D. A. (2009) ApcD is necessary for efficient energy transfer from phycobilisomes to photosystem I and helps to prevent photoinhibition in the cyanobacterium Synechococcus sp. PCC 7002. Biochim. Biophys. Acta, 1789: 1122-1128

Hou, H. J., Shen, G., Boichenko, V. A., Golbeck, J. H., and Mauzerall, D. (2009) Thermodynamics of Charge Separation of Photosystem I in the menA and menB Null Mutants of Synechocystis sp. PCC 6803 Determined by Pulsed Photoacoustics. Biochemistry 48: 1829-1837

Agalarov, R., Byrdin, M., Rappaport, F., Shen, G., Donald A. Bryant, D. A., van der Est, A., and Golbeck, J. H. (2008) Removal of the PsaF Polypeptide Biases Electron Transfer in Favor of the PsaB Branch of Cofactors in Triton X-100 Photosystem I Complexes from Synechococcus sp. PCC 7002. Photochemistry and Photobiology 84: 1371-1380.

Jin, Z., Heinnickel, M., Krebs, C., Shen, G., Golbeck, J. H., and Bryant, D. A. (2008) Biogenesis of iron-sulfur clusters in photosystem I: Holo-NfuA from the cyanobacterium Synechococcus sp. PCC 7002 rapidly and efficiently transfers [4Fe-4S] clusters to apo-PsaC in vitro. J. Biol. Chem. 283: 28426-28435
Shen, G., Leonard, H. S., Schluchter, W. M., and Bryant, D. A. (2008) CpcM Posttranslationally Methylates Asparagine-71/72 of Phycobiliprotein Beta Subunits in Synechococcus sp. Strain PCC 7002 and Synechocystis sp. Strain PCC 6803. J. Bacteriol. 190: 4808-4817.

Miller, C. A., Leonard, H. S., Pinsky, I. G., Turner, B. M., Williams, S. R., Harrison, Jr., L., Fletcher, A. F., Shen, G., Bryant, D. A., and Schluchter, W. M. (2008) Biogenesis of Phycobiliproteins: III. CpcM is the asparagine methyltransferase for phycobiliprotein β-subunits in cyanobacteria. J. Biol. Chem. 283: 19293-19300.
Shen, G., Schluchter, W. M., and Bryant, D. A. (2008) Biogenesis of phycobiliproteins. I. cpcS and cpcU mutants of the cyanobacterium Synechococcus sp. PCC 7002. Identify a phyocobiliprotein lyase specific for Cys-82/84 sites of the β-phycocyanin and allophycocyanin subunits. J. Biol Chem., 283: 7503-7512.

Krzystyniak, M., Shen, G., Golbeck, H. J., and Antonkine, M. L. (2008) Investigation of water bound to photosystem I with multiquantum filtered 17O nuclear magnetic resonance. J. Chem. Phys. 128 (11): 01450.

Shen, G., Balasubramanian, R., Wang, T., Wu, Y., Lee M. Hoffart, L. M., Krebs, C., Donald A. Bryant, A. D., and John H. Golbeck, J. H. (2007) SufR coordinates two [4Fe-4S]2+, 1+ clusters and functions as a ranscriptional repressor of the sufBCDS operon and an autoregulator of sufR in cyanobacteria. J. Biol. Chem. 282: 31909 – 31919.

Heinnickel, M., Shen, G. and Golbeck, J. H. (2007) Identification and Characterization of PshB, the Dicluster Ferredoxin that Harbors the Terminal Electron Acceptors FA and FB in Heliobacterium modesticaldum. Biochemistry, 46: 2530-2536.

Nomura, C., Persson, S., Shen, G., Inoue-Sakamoto, K., and Bryant, A. D. (2006) Charaterization of two cytochrome oxidase operons in the marine cyanobacterium Synechococcus sp. PCC 7002: Inactivation of ctaD1 affects the PS I:PS II ratio. Photosyn. Res. 87: 215-228.

Shen, G. and Golbeck, J. H. (2006) Chapter 31: Assembly of the bound iron-sulfur clusters in the photosystem I. In J. H. Golbeck (ed), Photosystem I: The plastocyanin (cytochrome c6)/ferredoxin (flavodoxin) oxidoreductase in photosynthesis. Pp529-548, Kluwer Academic Publishers, Dordrecht, The Netherland.

Shen, G., Saunée, N. A., Williams, S. R., Gallo, E. F., Schluchter, W. M., and Bryant, D. A. (2006) Identification and Characterization of a New Class of Bilin Lyase: The cpcT Gene Encodes a Bilin Lyase Responsible for Attachment of Phycocyanobilin to Cys-153 on the β-subunit of Phycocyanin in Synechococcus sp. PCC 7002. J. Biol. Chem. 281: 17768-17778

Balasubramanian, R., Shen, G., Bryant, D. A., and Golbeck, J. H. (2006) Regulatory Roles for IscA and SufA in Iron Homeostasis and Redox Stress Responses in the Cyanobacterium Synechococcus sp. PCC 7002. J. Bacteriol. 188: 3182-3191.

Heinnickel,M., Shen, G., Agalarov, R., and Golbeck, J. H. (2005) Resolution and reconstitution od a bound Fe-S protein from the photosynthetic reaction centers from Heliobacterium modesticaldum. Biochemistry, 44: 9950-9960.

Sakuragi, Y., Zybailov, B., Shen, G., Bryant, D. A., Golbeck, J. H., Diner, B. A., Karygina, I., Pushkar, Y., and Stehlik, D. (2005) Recruitment of a foreign quinone into the A1 site of photosystem I. Characterization of a menB rubA double deletion mutant in Synechococcus sp. PCC 7002 devoid of FX, FA, and FB and containing plastoquinone or exchanged 9,10-anthraquinone. J. Biol Chem 280: 12371-12381.

Shen, G., Balasubramanian, R., Wang, T., Tirupati, B., Bollinger, J. M., Golbeck, J. H., and Bryant, D. A. (2005) Functional Genomics of genes for the biogenesis of Fe/S proteins in cyanobacteria. In A. van der Est & D. Bruce (Eds) Photosynthesis: Fundamental Aspects to Global Perspectives. Vol II, pp 882-884, Alliance Communication Group Publisher.

Balasubramanian, R., Wang, T., Shen, G., Hoffart, L. M., Krebs, C., Golbeck, J. H., and Bryant, D. A. (2005) SufR is a [4Fe-4S] protein that functions as . In A. van der Est & D. Bruce (Eds) Photosynthesis: Fundamental Aspects to Global Perspectives. Vol I, pp 66-68, Alliance Communication Group Publisher.

van der Est, A., Kandrashkin, Y., Shen, G., and Golbeck, J. H. (2005) Alteration of phenyalanine PsaB F669 slows electron transfer in the PsaA branch of PS I. In A. van der Est & D. Bruce (Eds) Photosynthesis: Fundamental Aspects to Global Perspectives. Vol II, Pp 882-884, Alliance Communication Group Publisher.

Heinnickel, M., Shen, G., Agalarov, R., and Golbeck, J. H. (2005) The photosynthetic reaction center of Heliobacterium modesticaldum. In A. van der Est & D. Bruce (Eds) Photosynthesis: Fundamental Aspects to Global Perspectives. Vol I, pp 57-59, Alliance Communication Group Publisher

Cohen, R. O., Shen, G., Golbeck, J. H., Xu, W., Chitnis, P. R., Valieva, A., I., van der est, A., Pushkar, Y., and Stehlik, D. (2004) Evidence for asymmetric electron transfer in cyanobacterial photosystem I: analysis of a methionine-to-Leucine mutation of the ligand too the primary electron acceptor Ao. Biochemistry 43: 4741-4754.
van der Est, A., Valieva, A. I., Kandrashkin, Y. E., Shen, G., Donald A. Bryant, D. A., and John H. Golbeck , J. H. (2004) Removal of PsaF alters forward electron transfer in photosystem I: evidence for fast reoxidation of QK-A in subunit deletion mutants of Synechococcus sp. PCC 7002. Biochemistry 43: 1264-1275.

Wang, T., Shen, G., Balasubramanian, R., McIntosh, L., Bryant, D. A., and Golbeck, J. H. (2004) The sufR gene (sll0088) in Synechocystis sp. PCC 6803) functions as a repressor of the sufBCDS operon in iron-sulfur cluster biogenesis in cyanobacteria. J. Bacteriol. 186: 956-967

Gómez-Lojero, C., Pérez-Gómez, B., Shen, G., Wendy M. Schluchter, W. M., and Bryant, D. A. (2003) Interaction of Ferredoxin:NADP+ Oxidoreductase with Phycobilisomes and Phycobilisome Substructures of the Cyanobacterium Synechococcus sp. Strain PCC 7002. Biochemistry 42: 13800-13811.
Wu, X., Chitnis, P., Pushkar, J., Teutloff, C., Zech, S. G., Bittl, R., Dietmar Stehlik, D., Valieva, A., van der Est, A., Zybailov, B., Shen, G., and John H. Golbeck (2003) Electron transfer in cyanobacterial photosystem I. I. Physiological and spectroscopic characterization of site-directed mutants in a putative electron transfer pathway from A0 through A1 to FX. J. Biol. Chem. 278: 27864-27875.

Wu, X., Parag R. Chitnis, P., Alfia Valieva, A., van der Est, A., Brettel, K., Guergova-Kuras, M., Julia Pushkar, J., Zech, S. G., Stehlik, D., Shen, G., Zybailov, B., and Golbeck, J. H. (2003) Electron Transfer in cyanobacterial photosystem I. II. Determination of forward electron transfer rates of site-directed mutants in a putative electron transfer pathway from A0 through A1 to FX. J. Biol. Chem. 278: 27876-27887.

Yu, J., Shen, G., Wang, T., Bryant, D. A., McIntosh, L., and Golbeck, J. H. (2003) Suppressor mutations used to study photosystem I Biogenesis: sll0088 is a Novel Regulatory Gene Involved in reaction center accumulation and activity in Synechocystis sp. PCC 6803. J. Bacteriol. 185: 3878-3887.

Shen, G., Mikhail, M. L., van der est, A., Vassiliev, I. R., Brettel, K., Bittl, R., Zech, S. G., Zhao, J., Stehlik, D., Bryant, D. A., and Golbeck, J. H. (2002) Assembly of Photosystem I: II. Rubredoxin is required for the in vivo assembly of FX in Synechococcus sp. PCC 7002 as shown by optical and spectroscopy. J. Biol Chem.277: 20355-20366.

Shen, G., Zhao, J., Reimer, S. K., Antonkine, M. L., Cai, Q., Weiland, S. M., Golbeck, J. H., and Bryant, D. A. (2002) Assembly of Photosystem I: I. Inactivation of the rubA gene encoding a membrane-associated rubredoxin in the cyanobacterium Synechococcus sp. PCC 7002 causes a loss of photosystem I activity. J. Biol. Chem. 277: 20343-20354.

Sakuragi, Y., Zybailov, B., Shen, G., Jones, A. D., Chitnis, P. R., van der Est, A., Bittl, R., Zech, S., Stehlik, D., Golbeck, J. H., and Bryant, D. A. (2002) Insertional inactivation of the menG gene, encoding 2-phytyl-1,4-naphthoquinone methyltransferase of Synechocystis sp. PCC 6803, results in the incorporation of 2-phytyl-1,4-naphthoquinone into the A1 site and alteration of the equilibrium constant between A1 and FX in photosystem I. Biochemistry 41: 394-405.

Zhao, J., Shen, G., and Bryant, D. A. (2001) Photsystem stoichiometry and state transition in a mutant of the cyanobacterium Synechococcus sp. PCC 7002 lacking phycocyanin. Biochimica et Biophysica Acta 1505:248-257.

Antonkine, M. L., Bentrop, D., Bertini, I., Luchinat, C., Shen, G., Bryant, D. A., Stehlik, D., and Golbeck, J. H. (2000) Paramagnetic 1H NMR spectroscopy of the reduced, unbound photosystem I subunit PsaC: sequence specific assignment of contact-shifted resonances and identification of mixed and equal valence Fe-Fe pairs in [4Fe-4S] centers FA- and FB-. J. Bio. Inorgan. Chem., 5: 381-392.

Semenov, A. Y., Mamedov, M. D., Vassiliev, I. R., Zybailov, B., Shen, G., van der est, A., Stehlik, D., Diner, B. A., Chitnis, P. R., and Golbeck, J. H. (2000) Recruitment of a foreign quinone into the A1 site of photosystem I. III. Altered kinetics of electron transfer in phylloquinone biosynthetic pathway mutants studied by time-resolved optical, EPR and electrometric techniques. J. Biol. Chem., 275: 23429-23438.

Zybailov, B., Art can der Est, A., Zech, S., Teutloff, C., Johnson, W., Shen, G., Bittl, R., Stehlik, D., Chitnis, P.R., and Golbeck, J. H. (2000) Recruitment of a foreign quinone into the A1 site of photosystem I. II. Structural and functional characterization of phylloquinone biosynthetic pathway mutants by EPR and ENDOR spectroscopy. J. Biol. Chem., 275, 8531-8539.

Shen, G., Johnson, T. W., Zybailov, B., Kolling, D., Reategui, R., Beauparlant, S., Vassiliev, I. Bryant, D. A., Jones, A. D., Golbeck, J. H., and Chitnis, P. R. (2000) Recruitment of a foreign quinone into the A1 site of photosystem I. 1, Generic and physiological characterization of mutants in the phylloquinone biosynthetic pathway of Synechocystis sp. PCC 6803. J. Biol. Chem., 275, 8523-8530.

Mayer, K. L., Shen, G., Bryant, D. A., Locomte, J. T., and Falzone, C. J. (1999) The solution structure of photosystem I accessory protein E from the cyanobacterium Nostoc sp. Strain PC 8009. Biochemistry, 38, 13736-13746.

Shen, G., Antonkine, M. L., Vassiliev, I. R., Golbeck, H. J., and Bryant, D. A. (1998). A new gene encoding in rubredoxin-like protein have an important function in assembly of three Fe-S clusters in PS I. In Garab. G. (ed), Photosynthesis: Mechanisms and Effects, Vol IV, pp3147-3150, Kulmer Academic Publishers.

Zybailov, B., Shen, G., Vassiliev, I., Chitnis, P., Bryant, B. D., Reategui, R., Johnson, W., Chitnis, P. R., and Golbeck, J. H. (1998) Mutations in the phylloquinone biosynthetic pathway affect the properties of A1 in photosystem I. In Garab, G. (ed), Photosynthesis: Mechanics and Effects, Vol I, pp647-650, Kulmer Academic Publishers.

Reategui, R., Johnson, W., Xu, W., Zybailov, B., Vassiliev, I., Shen, G., Golbeck, J. H., and Chitnis, P. R. (1998) Mutational analysis of photosystem I: Function of phylloquinones. In Garab, G. (ed), Photosynthesis: Mechanisms and Effects, Vol I, pp515-520, Kulmer Academic Publishers.

Yang, F., Shen, G., Schluchter, W., Zybailov, B., Ganago, A., Vassiliev, I., Bryant, D., and Globeck, J. (1998) Deletion of the PsaF polypeptide modifies the environment of the redox-active phylloquinone *A1). Evidence for unidirectionality of electron transfer in photosystem I. J. Phys. Chem. 102, 8288-8299.

Yang, F., Shen, G., Schlutchter, W. A., Golbeck, J. H., and Bryant, D. A. (1998) Structural and functional analyses of cyanobacterial photosystem I: The directionality of electronic transfer. In Peschek, G. A., Loeffelhardt, W., Schmetterer, G. (Eds) The Photosynthetic Prokaryotes. Pp21-34. Kluwer Academic/Plenum Publishers.

Chung, S., Shen, G., Ormerod, J., and Bryant, D. A. (1998) Insertional inactivation of the csmA and csmC genes of the green sulfur bacterium Chlorobium virioforme 8327: the chlorosome protein CsmA is required for viability but CsmC is dispensable. FEMS Microbiol. Lett. 164: 353-361.

Sakamoto, T. Shen, G., Higashi, S., Murata, N., and Bryant, D. A. (1998) Alteration of low-temperature susceptibility of the cyanobacterium Synechococcus sp. PCC 7002 by genetic manipulation of membrane lipid unsaturation. Arch. Microbiol., 169: 20-28.

Schluchter, W. M., Shen, G., Zhao, J. D., and Bryant, A. B. (1996) Characterization of psal and psaL mutants of Synechococcus sp. Strain PCC 7002: A new model for state transitions on cyanobacteria. Photochem. And Photobiol., 64: 53-66.

Vermaas, W. F. J., Shen, G., and Ohad, I. (1996) Chimaeric CP47 mutants of the cyanobacterium Synechocystis sp. PCC 6803 carrying spinach sequences: construction and function. Photosyn. Res., 48: 147-162.

Shen, G., and Bryant, D. A. (1995) Characterization of a Synechococcus sp. Strain PCC 7002 mutant lacking photosystem I. Protein assembly and energy distribution in the absence of the photosystem I reaction center core complex. Photosyn. Res. 44: 41-53.

Shen, G. and Vermaas, W. F. J. (1994) Mutation of chlorophyll ligands in the chlorophyll-binding CP47 protein as studied in Synechocystis sp. PCC 6803 photosystem I-less background. Biochemistry 33: 7379-7388.

Shen, G. and Vermaas, W. F. J. (1994) Chlorophyll in a Synechocystis sp. PCC 6803 mutant without photosystem I and photosystem II core complexes. J.Biol. Chem. 269: 13904-13910.

Vermaas, W. F. J., Shen, G., and Styring, S. (1994) Electrons generated by photosystem II are utilized by and oxidase in the absence of photosystem I in the cyanobacterium Synechocystis sp. PCC 6803. FEBS Letters 337: 103-108.

Shen, G., Boussiba, S., and Vermaas, W. F. J. (1993) Synechocystis sp. PCC 6803 strains lacking photosystem I and phycobilisome function. The Plant Cell 5: 1853-1863.

Shen, G., Eaton-Rye, J., and Vermaas, W. F. J. (1993) Mutations of histidine residues in CP 47 leads to destabilization of the PS II complex and to impairment of light energy transfer. Biochemistry 32: 5109-5155.

Ikeuchi, M., Eggers, B., Shen, G., Webber, A. N., Yu, J., Hirano, A. Inoue, Y., and Vermaas, W. F. J. (1991) Cloning of the psbK gene from Synechocystis sp. PCC 6803 and characterization of photosystem II in mutants lacking PS II-K. J. Biol. Chem. 26: 11111-11115.

Vermaas, W. F. J., Charité, J., and Shen, G. (1990) Glu-69 of the D2 protein in photosystem II is potential ligand to Mn involved in photosynthetic oxygen evolution. Biochemistry 29: 5325-5332.

Vermaas, W. F. J., Charité, J., and Shen, G. (1990) QA binding to D2 contributes to the functional and structural integrity of photosystem II. Z. Naturforsch 45c: 359-365.

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