-For Drosophila Micro-injection-
Using the needle puller in Rick Ordway's lab: office: 417 Mueller, lab: 409 Mueller
Capillary Tubes: Sutter Instrument Co.
Cat. # BF100-78-10, OD = 1 mm, ID = 0.78 mm
40 Leveroni Court
Novato, CA 94949
phone: (415) 883-0128
fax: (415) 883-0572
Directions
NEVER CONTACT FILAMENT!!
1. Turn on needle puller (Power button on lower left side of machine).
2. Set program:
Program 3: Heat 615, Pull 150, Vel. 100, Time 1753. Using tweezers, get a glass capillary tube. Do not touch the center of the cap. tube (this is the part that will contact the embryo during injections), instead only contact the extreme edges.
Program 4: Heat 600, Pull 150, Vel. 100, Time 175
(Program 4 produces longer, finer needles)
4. Slide the tube into the clamp on the R side.
5. Tighten the clamp until the tube can slide R & L, but not up & down.
6. Slide the tube left until its right end is approximately 1/2 cm past the edge of the _____ (I don't what to call it)
7. Tighten the R knob so the tube to be secure, but not tight enough to break the tube.
8. Release the weights from the clamps.
9. Pull the two halves of the puller together by squeezing the handles at the bottom of the machine.
11. Tighten the L knob.
12. Pull the needles by pressing the 'pull' button.
13. Change the program by pressing the 'reset' button underneath the display panel.