Storage box form

Box: Oligo B
Location: Enzyme Freezer
1) DSIF1 0.1 mM	2) DSIF2 0.1 mM	3) DSIF1B 0.1 mM	4) DSIF2B 0.1 mM	5) DSIF3B 0.1 mM	6) DSIF4B 0.1 mM	7) TR4 0.1 mM	8) TR5 0.1 mM	9) TR6 0.1 mM
10) TRE-A 0.1mM	11) TRE-B 0.1mM	12) TRE-C 0.1mM	13) 70ter-1 0.1mM	14) 70ter-2 0.1mM	15) 70ter-3 0.1mM	16) GA-II 0.1mM	17 HSE-2 1.0 mM	18 T7 promoter
19 T7 terminator	20 DSIF 5'-2	21 NELF-A 5'-2	22 NELF-B 5'-2	23 NELF-C/D 5'-2	24 70ter-4	25 70ter-5	26 70ter-6	27 Pre TATA
28 HSF-1	29 HSF-2	30 GAGA-1	31 GAGA-2	32 GAGA-3	33 GAGA-4	34 TRE3	35 TRE4	36 PreTATA-2
37 Linker A'	38 Linker B	39)DBD2 (30) {Hokuto's Gal4)	40)DBD2 (30) {Hokuto's Gal4)	41)DBD1 (32) {Hokuto's Gal4}	42)DBD1 (32) {Hokuto's Gal4}	43 hsGal4-1A	44 hsGal4-1B	45 hs-Sp1-1
46 HT1	47 HT2	48 HT3	49 G1	50 G2	51 HSP-T aka hs-TR	52 TRE-1	53 TRE-2	54) SV40 ter.1
55 TRE-N/Z1	56 TRE-N/Z2	57 TRE-N/Z3	58 Zif268//NRE-1	59 Zif268//NRE-2	60 VP16-1	61 VP16-2	62) Gal4 DBD 3	63)
64 his-TATptd-1	65 his-TATptd-2	66 his-TATptd-3	67)	68)	69)	70)	71)	72)
73)	74)	75)	76)	77)	78)	79)	80)	81)

Description of contents:

Row 1 (return to top)
1)	DSIF1: CAAAAGGAGGACGAGATAGAGGAGT, 0.1mM in TE, prepared by LifeTechnologies 8/20/99, Primer number M8215B05
2)	DSIF2: CAAGGCTCCTCGCATGCGTGTGTAG, 0.1mM in TE, prepared by LifeTechnologies 8/20/99, Primer number M8215B06
3)	DSIF 1B: GGTCTTGCATATGCAAAAGGAGGACGAGATAGAGGAGT, 0.1mM in TE, prepared by LifeTechnologies 9/20/99, Primer number M9095G02
4)	DSIF 2B: GGAATTCTACAAGGCTCCTCGCATGCGTGTGTAG, 0.1mM in TE, prepared by LifeTechnologies 9/20/99, Primer number M9095G03
5)	DSIF 3B: CCAGGCTAGCCAGAATGTGGGTGTCATAGTGCGTTTG, 0.1mM in TE, prepared by LifeTechnologies 9/20/99, Primer number M9095G04
6)	DSIF 4B: GGAATTCTACCTGGCCGGCGTTGTGTTTGCCGTG, 0.1mM in TE, prepared by LifeTechnologies 9/20/99, Primer number M9095G05
7)	TR4:CTTCTGGTGCCGGAAACCAG, 0.1mM in TE, prepared by LifeTechnologies 9/1/99, for use in LM-PCR analysis of +100 to +200 region.
8)	TR5:GCCGGAAACCAGGCAAAGCG, 0.1mM in TE, prepared by LifeTechnologies 9/1/99, for use in LM-PCR analysis of +100 to +200 region.
9)	TR6:CCAGGCAAAGCGCCATTCGCC, 0.1mM in TE, prepared by LifeTechnologies 9/1/99, for use in LM-PCR analysis of +100 to +200 region.

Row 2 (return to top)
10)	TRE-A: AAGTCGAGCTCGAGCG, 0.1mM in TE, prepared by LifeTechnologies 11/24/99, for use in LM-PCR analysis of TRE70w from just upstream of the TATA element.
11)	TRE-B: TCGAGCGCCGGAGTATAAATAG, 0.1mM in TE, prepared by LifeTechnologies 11/24/99, for use in LM-PCR analysis of TRE70w from just upstream of the TATA element.
12)	TRE-C: GCCGGAGTATAAATAGAGGCGCTTC, 0.1mM in TE, prepared by LifeTechnologies 11/24/99, for use in LM-PCR analysis of TRE70w from just upstream of the TATA element.
13)	70ter-1: CGTCAGTATCGGCGGAATT, 0.1mM in TE, prepared by LifeTechnologies 11/15/99, for use in LM-PCR to analyze termination on our transgenes.
14)	70ter-2: TATCGGCGGAATTCCAGCTGA, 0.1mM in TE, prepared by LifeTechnologies 11/15/99, for use in LM-PCR to analyze termination on our transgenes.
15)	70ter-3: GAATTCCAGCTGAGCGCCGGTC, 0.1mM in TE, prepared by LifeTechnologies 11/15/99, for use in LM-PCR to analyze termination on our transgenes.
16)	GA-II: GATCCTGGCTCTCTGTTTC, 0.1mM in TE, prepared by LifeTechnologies 12/99, Liu plans on using it with GA-I to make GAGA factor competitor.
17)	HSE-2: CTAGAAGCTTCTAGAAGCTT, 1.0mM in TE (note high concentration), prepared by LifeTechnologies 12/99, Liu plans on using it to prepare a competitor for HSF.
18)	T7 promoter: TAATACGACTCACTATAGGG, 0.1mM in TE, prepared by LifeTechnologies 1/00, Chwen-Huey's sequencing in the DSIF/NELF project.

Row 3 (return to top)
19)	T7 terminator: GCTAGTTATTGCTCAGCGG, 0.1mM in TE, prepared by LifeTechnologies 1/00, Chwen-Huey's sequencing in the DSIF/NELF project.
20)	DSIF 5'-2: AGGTGGACTACGTGGATC, 0.1mM in TE, prepared by LifeTechnologies 1/00, Chwen-Huey's DSIF/NELF project.
21)	NELF-A 5'-2: AGACGATGAAGACCTTTCC, 0.1mM in TE, prepared by LifeTechnologies 1/00, Chwen-Huey's DSIF/NELF project.
22)	NELF-B 5'-2: GTCGTTCGGGTGAAGTCG, 0.1mM in TE, prepared by LifeTechnologies 1/00, Chwen-Huey's DSIF/NELF project.
23)	NELF-C/D 5'-2: TCGCTAATCTACCGACTGG, 0.1mM in TE, prepared by LifeTechnologies 1/00, Chwen-Huey's DSIF/NELF project.
24)	70ter-4: TTAATAATTTGCGAGTACGCA, 0.1mM in TE, prepared by LifeTechnologies 1/00, for LM-PCR analysis of termination on our transgenes.
25)	70ter-5: GAGTACGCAAAGCTTGGGCT, 0.1mM in TE, prepared by LifeTechnologies 1/00, for LM-PCR analysis of termination on our transgenes.
26)	70ter-6: AAAGCTTGGGCTGCAGGTCG, 0.1mM in TE, prepared by LifeTechnologies 1/00, for LM-PCR analysis of termination on our transgenes.
27)	Pre TATA: CGAATGTTCGCGAAAAGAGC, 0.1mM in TE, prepared by LifeTechnologies 4/00, for PCR reactions beginning immediately upstream from the TATA box of "normal" hsp70 promoters. Originally for Benjamin's comparison of TBP and TFIID footprints in IID depleted/rescued txn rxn.

Row 4 (return to top)
28)	HSF-1: CCCTAGGTACCTGGCCATTGATCAGA, 0.1mM in TE, prepared by LifeTechnologies 1/00, for amplifying the activation domain of HSF
29)	HSF-2: GGAATTCTAGAG TTACAACTCGTGACG, 0.1mM in TE, prepared by LifeTechnologies 1/00, for amplifying the activation domain of HSF
30)	GAGA-1: CCCTAGGTACCTGCCAATGAATTCG, 0.1mM in TE, prepared by LifeTechnologies 1/00, for amplifying the BTB/POZ domain of GAGA factor
31)	GAGA-2: GGAATTCTAGAGGTCCTTGGTAACCGT, 0.1mM in TE, prepared by LifeTechnologies 1/00, for amplifying the BTB/POZ domain of GAGA factor
32)	GAGA-3: CCCTAGGTACCCAACGAGAGTGATA, 0.1mM in TE, prepared by LifeTechnologies 1/00, for amplifying the Q domain of GAGA factor
33)	GAGA-4: GGAATTCTAGACTACTGCGGCTGCGG, 0.1mM in TE, prepared by LifeTechnologies 1/00, for amplifying the Q domain of GAGA factor
34)	TRE3: GCT CTA GAG TTT ACC ACT CCC TAT CAG TGA, prepared by Life Technologies 2/00, for preparing hsp70 with one TRE.
35)	TRE4: G CTC GAG CTC GAC TTT CAC TTT TCT CTA TCA CTG ATA GGG AGT, prepared by Life Technologies 2/00, for preparing hsp70 with one TRE.
36)	PreTATA-2: GAGAGCGCGCCTCGAATGTTC, prepared by Life Technologies 6/00, for PCR amplifying promoter fragments for TFIID binding experiments.

Row 5 (return to top)
37)	Linker A': GCG GTG ATT TAA AAG ATC TGA ATT C, prepared by Life Technologies 1/00, one of common oligos for ligation during LMPCR
38)	Linker B: GAA TTC AGA TC, prepared by Life Technologies 1/00, one of common oligos for ligation during LMPCR
39)	DBD2 (30): TAG CTA CGC GGA TCC TTA CTT ATC TAG AGC, prepared by Hokuto with Simpson's synthesized 8/99, used to make HS Gal4/Sp1A+B.
40)	DBD2 (30): TAG CTA CGC GGA TCC TTA CTT ATC TAG AGC, prepared by Hokuto with Simpson's synthesized 8/99, used to make hs Gal4 1-94 and hs Gal4 1-94/Sp1A+B.
41)	DBD1 (32): TAC GCG GAT CCA CAT ATG AAG CTA CTG TCT TC, prepared by Hokuto with Simpson's synthesized 8/99, used to make hs Gal4 1-94 and hs Gal4 1-94/Sp1A+B.
42)	DBD1 (32): TAC GCG GAT CCA CAT ATG AAG CTA CTG TCT TC, prepared by Hokuto with Simpson's synthesized 8/99, used to make hs Gal4 1-94 and hs Gal4 1-94/Sp1A+B.
43)	hsGal4-1A:CTGCAACTACTGAAATCTGCC,prepared by Life Technologies 9/99, for sequencing Hokuto's hs Gal4 1-94 and hs Gal4 1-94/Sp1A+B
44)	hsGal4-1B: CCCGACACCAGACCAACTG,prepared by Life Technologies 9/99, for sequencing Hokuto's hs Gal4 1-94 and hs Gal4 1-94/Sp1A+B
45)	hs-Sp1-1: CAG GAC TAC CTG GAG TGA TGC, prepared by Life Technologies 10/99, for sequencing Hokuto's hs Gal4 1-94/Sp1A+B

Row 6 (return to top)
46)	HT1: GCCCTCGAGCTGCAGGTCCTCGGA, prepared by Hongbing with Simpson's synthesizer, 30 nmoles/40 ul, used to amplify Gal4-UAS5.
47)	HT2: GGCGCTCTCTAGAGTCGGAGT, prepared by Hongbing with Simpson's synthesizer, 30 nmoles/40 ul, used to amplify Gal4-UAS5.
48)	HT3: ACTCTAGA GAGCGCCGGAGTATA, prepared by Hongbing with Simpson's synthesizer, 30 nmoles/40 ul, used to amplify hsp70 core and link it to Gal4 UAS.
49)	G1: GGCGCTCTCTAGAGTCGGAGTACTGTCCTCCGAGGACCTGCA, prepared by Hongbing with Simpson's synthesizer, 1 nmoles/1 ul, used to link a single Gal4-UAS to hsp70 core.
50)	G2: GGC GCT CTC TAG AGT CGG AGT ACT GTC CTC CGA GCG GAG TAC TGT CCT CCG AGG ACC TGC A, prepared by Hongbing with Simpson's synthesizer, 30 nmoles/40 ul, used to link two Gal4-UAS to hsp70 core.
51)	HSP-T: GAGCTCGAGCGCCGGAGTATA, prepared by Hongbing with Simpson's synthesizer, 30 nmoles/40 ul, used to amplify core hsp70 core and link it to TRE elements.
52)	TRE-1: GGCCCTTTCGTCCTCGAGTT, prepared by Hongbing with Simpson's synthesizer, 30 nmoles/40 ul, used to amplify TRE elements and link to core hsp70 core.
53)	TRE-2: TCCGGCGCTCGAGCTCGACTT, prepared by Hongbing with Simpson's synthesizer, 30 nmoles/40 ul, used to amplify TRE elements and link to core hsp70 core.
54)	SV40 ter.1: ttgtccaattatgtcacacca, from IDT 7/14/03, anneals to the SV40 terminator in pUAST and used to sequence insert.

Row 7 (return to top)
55)	TRE-N/Z1: CCCTTCTTACCGCCCACGCCTGAACCCTTGCAGACTCGAGCTCGAC, from Life Technology, 6/00, for use in constructing the target for the Zif268//NRE DBD.
56)	TRE-N/Z2: GGGCGGTAAGAAGGGTTCAGGCGTGGGCGGAGCGCCGGAGTATAAAT, from Life Technology, 6/00, for use in constructing the target for the Zif268//NRE DBD.
57)	TRE-N/Z3: AAAGTCGAGCTCGAGTCTGC, from Life Technology on 9/5/00, for use in constructing the target for the Zif268//NRE DBD.
58)	Zif268//NRE-1: GTCGTGGCGGCCCACCTCCCAAAAAGAAAAGAAAAGTTG, from Life Technology, 6/00, for use in constructing Zif268//NRE-VP16.
59)	Zif268//NRE-2: tcgtacgcgcgcggctgtaGGTaccCTTCTGTCTTAAGTGG, from Life Technology, 6/00, for use in constructing Zif268//NRE-VP16.
60)	VP16-1: CCACTTAAGACAGAAGggtACCtacagccgcgcgcgtacgaa, from Life Technology, 6/00, for use in constructing Zif268//NRE-VP16.
61)	VP16-2: ctggtctagaCTAcccaccgtactcgtcaa, from Life Technology, 6/00, for use in constructing Zif268//NRE-VP16.
62)	Gal4 DBD 3: GAGACCTTTTGGTTTTGGGAGAGTAG, Use in conjunction with TRE C to detect Gal42-1 hs insert.
63)

Row 8 (return to top)
64)	his-TATptd-1: ATGCATCATCACCACCATCACGGCTACGGCCGTAAAAAGCG, from Life Technology, 6/00, for use in constructing Zif268//NRE-VP16.
65)	his-TATptd-2: GTGGGCCGCCACGACGACGCTGACGACGCTTTTTACGGCCG, from Life Technology, 6/00, for use in constructing Zif268//NRE-VP16.
66)	his-TATptd-3: acgcagatctATGCATCATCACCACCATC, from Life Technology, 6/00, for use in constructing Zif268//NRE-VP16.
67)
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Row 9 (return to top)
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