plasmids DJT

Box: plasmids DJT (from Deb Taxman)
Location: Lakshmi's fridge
1)pAR3038 (TBP exp.)	2) pAR GAGA	3) pAR GAGA	4) pAR GAGA	5) pET GAGA	6) pET GAGA	7) pET GAGA-*NcoI	8)pET GAGA-*NcoI	9) BlueScript pBSK+
10) pAR *RV	11) pAR *RV	12) pAR *RV	13)pET **GAGA	14)pET **GAGA	15)pET **GAGA	16) pET-dRD Yamaguchi	17) p70w	18) p70w delta TA
19 pTet-ON (clonetech)	20 pUHD15.1 (Clonetech)	21 pUHD10.3	22	23	24 pCaSpeR-hs Tet-off Vp16	25 pCaSpeR-hs Tet-On Vp16	26) T7 hsp70	27) pUC13
28) mGA-50 Car20 (ZT.2 derivative)	29)Car20 -50/+84 (ZT.2 derivative)	30)Car20 -50/+84 (ZT.2 derivative)	31)Car20 -89/+84 (ZT.2 derivative)	32)Car20 -89/+84 (ZT.2 derivative)	33)Car20 -190/+84 (ZT.2 derivative)	34)Car20 -190/+84 (ZT.2 derivative)	35) H3 S-X Car20 (ZT.2 derivative)	36) H3 *S-X Car20 (ZT.2 derivative)
37) mGAGA -50/+84 pUCXBS	38) hsp70 *9	39 -190 pUC13 -2C to G	40) -190 pUC13 -30/-2	41) -190 pUC13 *TATA	42) -190 pUC13 wildtype	43) -50 to +89	44) H3/H4 S/X	45 pRP 49 0.66 ug/ul
46 G1 70w	47 G2 70w	48 G5 70w	49 TRE1 70w	50 TRE5 70w	51 G1 70z	52 G2 70z	53 G5 70z	54 TRE5 70z
5 pTß stop TAF30beta	56 pTß stop TAF40	57 pTß stop TAF60	58 pTß stop TAF80	59 pTß stop TAF110	60 pTß stop TAF150	61 pTß stop TAF250	62 pCS-268//NRE	63 pCR102 "rp49"clone
64 pCaSpeR-hs Gal4 GAGA POZ	65 pCaSpeR-hs Gal4 GAGA Q	66 pCaSpeR-hs Gal4 HSF	67 pCS-268//NRE	68 pTAT (protein transduction)	69 pTAT-HA (protein transduction)	70 pNO TAT beta gal in cells	71 pTAT-HA-beta gal in cells	72 pTATA-GFP in cells
73 pCaSpeR-hs Gal4 1-94	74 pCaSpeR-hs Gal4 Sp1 A&B	75 pCaSpeR-hs	76 pCaSpeR-hs 268//NRE-VP16	77 pCS-268//NRE	78 pCS-268//NRE VP16	79 pGEM TRE5 70	80 pGEM TRE5 70 N/ZZ/N 70	81)pCR102 "rp49"clone

Description of contents:

Row 1 (return to top)
1)	pAR3038, PET vector for producing Drosophila TBP.
2)	pAR GAGA
3)	pAR GAGA
4)	pAR GAGA
5)	pET GAGA
6)	pET GAGA
7)	pET GAGA-*NcoI, N-terminal deletion that removes a.a. 1 - 279, retains zinc finger (aa 343-366) and Gln-rich domain.
8)	pET GAGA-*NcoI
9)	BlueScript (pBSK+) from Thomas lab, 250 ng/ul, 20 ul total.

Row 2 (return to top)
10)	pAR *RV, C-terminal deletion that removes a.a. 425-518
11)	pAR *RV
12)	pAR *RV
13)	pET **GAGA, N and C-terminal deletions removing a.a. 1-279 and 425-518.
14)	pET **GAGA
15)	pET **GAGA
16)	pET-dRD, 60ng/ul, from Y. Yamaguchi. Encodes 38 kD polypeptide of anonymous 66Da, a putative subunit of NELF
17)	p70w. 450 ng/ul Prepared by Chris Bell. promoter in the white vector.
18)	p70w delta TA. 490 ng/ul Prepared by Chris Bell. promoter in the white vector.

Row 3 (return to top)
19)	pTet-ON (Clonetech)
20)	pUHD15.1 (Clonetech)
21)	pUHD10.3 (Clonetech)
22)
23)
24)	pCaSpeR-hs Tet-off Vp16, made by Vivian Wang Sp00
25)	pCaSpeR-hs Tet-On Vp16, made by Vivian Wang Sp00
26)	T7 hsp70. 175 ng/ul. This was prepared by Larry Benjamin and contains the T7 promoter adjacent to the hsp70 transcription start.
27)	pUC13

Row 4 (return to top)
28)	mGA-50 Car20 (ZT.2 derivative), Note that the DNA preparations in slots 28 to 36 represent the original collection that Jan Weber used in her work.
29)	Car20 -50/+84 (ZT.2 derivative)
30)	Car20 -50/+84 (ZT.2 derivative)
31)	Car20 -89/+84 (ZT.2 derivative)
32)	Car20 -89/+84 (ZT.2 derivative)
33)	Car20 -190/+84 (ZT.2 derivative)
34)	Car20 -190/+84 (ZT.2 derivative)
35)	H3 S-X Car20 (ZT.2 derivative)
36)	H3 *S-X Car20 (ZT.2 derivative)

Row 5 (return to top)
37)	mGAGA -50/+84 pUCXBS
38)	hsp70 *9, I think this is one of Beverly Purnell's deletions at the Acc1 site. It shortens the spacing between the TATA box and the initiator.
39)	-190 pUC13 -2C to G
40)	-190 pUC13 -30/-2
41)	-190 pUC13 *TATA
42)	-190 pUC13 wildtype
43)	-50 to +89
44)	H3/H4 S/X
45)	pRP 49: provide by Kay, Roshbash's original clone.

Row 6 (return to top)
46)	G1 70w
47)	G2 70w
48)	G5 70w
49)	TRE1 70w
50)	TRE5 70w
51)	G1 70z
52)	G2 70z
53)	G5 70z
54)	TRE5 70z

Row 7 (return to top)
55)	pTß stop TAF30beta: plasmid made in Tjian lab and provided by David Wassarman at NIH.
56)	pTß stop TAF40: plasmid made in Tjian lab and provided by David Wassarman at NIH.
57)	pTß stop TAF60: plasmid made in Tjian lab and provided by David Wassarman at NIH.
58)	pTß stop TAF80: plasmid made in Tjian lab and provided by David Wassarman at NIH.
59)	pTß stop TAF110: plasmid made in Tjian lab and provided by David Wassarman at NIH.
60)	pTß stop TAF150: plasmid made in Tjian lab and provided by David Wassarman at NIH.
61)	pTß stop TAF250: plasmid made in Tjian lab and provided by David Wassarman at NIH.
62)	pCS-268//NRE
63)	pCR102: ro49 clone from Cavener

Row 8 (return to top)
64)	pCaSpeR-hs Gal4 GAGA POZ: aka hsGal4 GAGA POZ, 50 ul at 0.68 ug/ul
65)	pCaSpeR-hs Gal4 GAGA Q: aka hsGal4 GAGA Q, 50 ul at 1.3 ug/ul
66)	pCaSpeR-hs Gal4 HSF: aka hsGal4 HSF, 50 ul at 1.2 ug/ul
67)	pCS-268//NRE: high affinity zinc finger protein from Jin-Soo Kim (for PSU seed grant)
68)	pTAT from Steven Dowdy for use in protein transduction (for PSU seed grant)
69)	pTAT-HA from Steven Dowdy for use in protein transduction (for PSU seed grant)
70)	pNO TAT beta gal in E. coli, from Steven Dowdy for use in protein transduction (for PSU seed grant)
71)	pTAT-HA-beta gal in E. coli, from Steven Dowdy for use in protein transduction (for PSU seed grant)
72)	pTATA-GFP in E. coli, from Steven Dowdy for use in protein transduction (for PSU seed grant)

Row 9 (return to top)
73)	pCaSpeR-hs Gal4 1-94, made by Hokuto Nishioko, 1999 (780 ng/ul)
74)	pCaSpeR-hs Gal4 Sp1 A&B, made by Hokuto Nishioko, 1999 (687 ng/ul)
75)
76)
77)
78)
79)
80)	pCS-268//NRE: Miniprep DNA by DSG
81)	0.6 kb H3/PstI fragment from rp49 inserted into Bluescript KS(+). Obtained from Doug Cavener. Miniprep DNA by DSG

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