Box: Plasmids DSG-Z
Location: Lakshmi's Fridge
1) 
H3/H4 S/X		 2)
H4 pUC13		 3)
Hsp26 S/X		 4)
88BE		 5)
26pr pUC9		 6)
88XbE		 7) 8)
pUC13		 9)
pUC13
10)
H3/H4 S/X8		 11)
Hsp26 S/X6		 12) hsp70wt
XBS		 13)*TATA
hsp70
XBS		 14) *TATA XBS
1.5 mg/ml		 15) 16) 17)
G5E4T		 18)
G1E4T
19)
CaSpeR 4		 20)
CaSpeR 4		 21)
pW8 T.H.SV40		 22)
pC.T.H.Z.A3		 23)
Helper DNA 		 24)
CaSpeR		 25)
LacZT		 26)
G5E4T		 27)
G1E4T
28)
Car20ZT.2 -194//+84		 29)
Car20ZT.2 -194/*TATA/+84		 30)
Car20ZT.2 -194/-30G/+84		 31)Car20ZT.2 -194/-30G,
-2G/+84		 32)
Car20ZT.2		 33)
-2G/XBS		 34)
-2G,-30G/XBS		 35)
-30G/XBS		 36)
LacZT
37)
hsp70/87C		 38)
G2 70W		 39)
G5 70W		 40)
G1 70W		 41)
TRE 70W		 42)
TRE6 70Z		 43)
G1 70Z		 44)
G2 70Z		 45)
G5 70Z
46)
hsp70 wt 		 47)
hsp70 *TATA		 48)
hsp70 CCCA		 49)
hsp70 Inr		 50)
hsp70 +14 to +19		 51)
hsp70 +24 to +29		 52)
hsp70 +30 to +35		 53)
hsp70
Inr/+25 double mut.		 54)
55)
hsp70 wt 		 56)
hsp70 *TATA		 57)
hsp70 CCCA		 58)
hsp70 Inr		 59)
hsp70 +14 to +19		 60)
hsp70 +24 to +29		 61)
hsp70 +30 to +35		 62)
-2 XBS		 63)
-2, -30 XBS
64)
pTet Splice		 65)
pTet tTAK		 66)
pUHD15-1		 67)
pUHC13-3		 68)
pUHG17-1		 69)
pUHG17-1		 70)
pTRE		 71)
pUHD-10		 72)
73) WT 70W
(a.k.a. -194//+84 Z.T.W.)		 74)delta TA
70W (CaSpeR derivative)		 75) Inr 70W
(CaSpeR derivative)		 76) WT 70W 1ng/ul 77)
pP{CaSpeR-act(B)}		 78)
pP{CaSpeR-act(B)}		 79)
pP{CaSpeR-act(B)}		 80)
pUAST		 81)
pUAST

Description of contents:

Row 1 (return to top)
1)
  H3/H4 S/X: Despite the label, the concentration is unkown because the tube had dried out.
2)
  H4 pUC13: Spans H4 from about -50 to +100. 
3)
  Hsp26 S/X: Baiyong Li's preparation of the hsp26 S/X. 
4)
  88BE: hsp26 promoter from -170 to +7 cloned into pUC13; this is transcribed in vitro (MCB paper)
5)
  26pr pUC9: see note in strider file.  The orientation of the hsp26 promoter was never determined!
6)
  88XbE: hsp26 promoter from about -50 to +7 (Xba/EcoR1)
7)
  
8)
  pUC13: 7mg/ml
9)
  pUC13: 7mg/ml
Row 2 (return to top)
10)
  H3/H4 S/X8 -- contains 8 tandem copies of the H3/H4 promoter.
11)
  Hsp26 S/X6 -- contains 6 tandem copies of the hsp26 promoter.
12)
  hsp70 wt XBS: Mike Sypes purple tube at 7.1 mg/ml, -194 to +84, same as slot 46 in this box.
13)
  *TATA hsp70 XBS: Weber's prep at 680 ng/ul, -194 to +84 with TATA deletion, same as slot 47 in this box.
14)
  *TATA XBS, 1.5 mg/ml
15)
  
16)
  
17)
  G5E4T
18)
  G1E4T
Row 3 (return to top)
19)
  CaSpeR 4: Susan Abmayr's original preparation from Maniatis lab, 600ng/ul
20)
  CaSpeR 4: Miisy Errickson's preparation, 900ng/ul
21)
  pW8 T.H.SV40: From Bruno Bello, tet operator, hsp70 minimmal, SV40 polyA
22)
  pC.T.H.Z.A3: From Bruno Bello, rosy, tet operator, hsp70 minimal, lacZ
23)
  Helper DNA: From Bruno Bello, transposase helper plasmid but he didn't know its structure.
24)
  CaSpeR: Hongbing, this might be CaSpeR 4 because I don't recall working with CaSpeR.
25)
  LacZT: From Hongbing, 194 ng/ul
26)
  G5E4T
27)
  G1E4T
Row 4 (return to top)
28)
  Car20ZT.2 -194//+84 -- 1.6 ug/ul, Weber's prep., I checked on gel 5/97.
29)
  Car20ZT.2 -194/*TATA/+84: 2ug/ul, Weber's prep.
30)
  Car20ZT.2 -194/-30G/+84: tube is almost dry
31)
  Car20ZT.2 -194/-30G,-2G/+84: 5ug/ul
32)
  Car20ZT.2: source not known.
33)
  -2G/XBS: hsp70 derivative. Unknown concentration and some uncertainty whether this is XBS or pUC13. Hongbing made certain the internal RI site is present.
34)
  -2G,-30G/XBS: hsp70 derivative. Unknown concentration and some uncertainty whether this is XBS or pUC13. Hongbing made certain the internal RI site is present.
35)
  -30G/XBS: hsp70 derivative. Unknown concentration and some uncertainty whether this is XBS or pUC13. Hongbing made certain the internal RI site is not present.
36)
  LacZT: reconstituted from a dry tube, Hongbing was able to make good DNA by transformation.
Row 5 (return to top)
37)
 hsp70/87C: 1.8 ug/ul, subclone of the Xba 1 fragment from 132E3 into pUC13, see Weber’s thesis.
38)
  G2W: from Hongbing, aka G2 70W, for fly transformation
39)
  G5W: from Hongbing, aka G5 70W, for fly transformation
40)
  G1W: from hongbing, aka G1 70W, for fly transformation
41)
  TRE 70W: from Hongbing, for fly transformation
42)
  TRE6 70Z: Hongbing's construct based on rosy selection
43)
  G1 70Z: Hongbing's construct based on rosy selection
44)
  G2 70Z: Hongbing's construct based on rosy selection
45)
  G5 70Z: Hongbing's construct based on rosy selection
Row 6 (return to top)
46)
  hsp70 wt: from Sypes purple tube, 7.1 ug/ul (probably in XBS)
47)
  hsp70 *TATA: from Weber, 680 ng/ul (probably in XBS)
48)
  hsp70 CCCA: TATA mutant from Madabusi box 3 ? 700 ng/ul (#39) (probably in XBS)
49)
  hsp70 Inr: worst Inr from Madabusi box 3 ? unknown conc. (#20) (probably in XBS)
CGGAGTATAAATAGAGGCGCTTCGTCTACGGAGCGcacccTCAATTCAAACAAGCAAAGTGAACACGTCGCTAAGCGAAA
50)
  hsp70 +14 to +19: from Madabusi box 3, 330 ng/ul (#30) (probably in XBS)
CGGAGTATAAATAGAGGCGCTTCGTCTACGGAGCGACAATTCAATTCAAACttaCttAGTGAACACGTCGCTAAGCGAAA
51)
  hsp70 +24 to +29: original +24 mutant from Madabusi box 3. unknown conc. (#8) (probably in XBS)
CGGAGTATAAATAGAGGCGCTTCGTCTACGGAGCGACAATTCAATTCAAACAAGCAAgaattcCACGTCGCTAAGCGAAA
52)
  hsp70 +30 to +35: from Bell, prepared by fall 97 rotations, unknown conc. and dirty. (probably in XBS)
CGGAGTATAAATAGAGGCGCTTCGTCTACGGAGCGACAATTCAATTCAAACAAGCAAAGTGAACACGcCtaggAGCGAAA
53)
  hsp70 Inr/+25 double mutatant: from Madabusi box 3, (#18).  CsCl prep at 492 ng/ul
54)
  
Row 7 (return to top)
55)
  hsp70 wt: from Sypes purple tube, 7.1 ug/ul (probably in XBS)
56)
  hsp70 *TATA: from Weber, 680 ng/ul (probably in XBS)
57)
  hsp70 CCCA: TATA mutant from Madabusi box 3, 700 ng/ul (#39) (probably in XBS)
58)
  hsp70 Inr: worst Inr from Madabusi box 3, unknown conc. (#20) (probably in XBS)
59)
  hsp70 +14 to +19: from Madabusi box 3, 330 ng/ul (#30) (probably in XBS)
60)
  hsp70 +24 to +29: original +24 mutant from Madabusi box 3, unknown conc. (#8).  Jan.99 - Lui has this tube. (probably in XBS)
61)
  hsp70 +30 to +35: from Bell, prepared by fall 97 rotations, unknown conc. and dirty. (probably in XBS)
62)
  -2 XBS: status is unknown.  hsp70 derivative
63)
  -2, -30 XBS: status is unknown. hsp70 derivative
Row 8 (return to top)
64)
  pTet Splice
65)
  pTet tTAK
66)
  pUHD15-1
67)
  pUHC13-3
68)
  pUHG17-1
69)
  pUHG17-1
70)
  pTRE
71)
  pUHD-10
72)
  
Row 9 (return to top)
73)
  WT 70W: part of rosy, the hsp70 promoter from -194 to +84, and beta gal all subcloned into CaSpeR 4. Also known as -194//+84 Z.T.W. (go here for a macplasmap image)
74)
  delta TATA 70W: part of rosy, the hsp70 promoter from -194 to +84 with TATA deletion, and beta gal all subcloned into CaSpeR 4.
75)
  Inr 70W: part of rosy, the hsp70 promoter from -194 to +84 with Lakshmi's Inr mutations, and beta gal all subcloned into CaSpeR 4.
76)
  WT 70W 1ng/ul
77)
  pP{CaSpeR-act(B)} Tansformation vector for expressing proteins via the actin promoter, Auerbach's prep.
78)
  pP{CaSpeR-act(B)} Tansformation vector for expressing proteins via the actin promoter, source unknown.
79)
  pP{CaSpeR-act(B)} Tansformation vector for expressing proteins via the actin promoter, source unknown.
80)
  pUAST provided by Graham Thomas.  Originally from Perrimon.
81)
  pUAST, Auerbach's preparation
(return to top)