Plasmids DSG 4B

Box: DSG 4B
Location: Lakshmi's Fridge in 450 N. Frear
Note that items in red are ones that are missing or are empty.
1)hsp70 -190 mp18	2hsp70 -89 mp18	3)hsp70 -50 mp18	4)pXM1	5)pHT1	6)pXM40	7)pXM40	8)hsp70 -190/ *TATA/ +89pUC13	9)-190 pUC13 -30/-2	10)hsp70 -190/ -30/+89 pUC13
11)hsp70 -190//+89 pUC13	12)hsp70 -190/ *TATA/ +89pUC13	13)hsp70 -190/-2/ +89 pUC13	14)hsp70 -190/-30/ +89 pUC13	15)hsp70 -190/-30, -2/+89 pUC13	16)hsp70 -190 with dU	17)hsp70 -89 with dU	18)hsp70 -50 with dU	19) hsp70 S/X-1	20) hsp70 S/X-1
21)-190 hsp70 wt mp18	22)-190 hsp70 *TATA mp18,	23)-190 hsp70-30 mutation mp18	24). -190 hsp70 -2 mutation mp18	25)-190 hsp70 -30,-2 mutation mp18	26)hsp70 +35// -12 S/X3	27)hsp70 +35// -12 S/X1	28) hsp70 +35//-12 S/X1	29)hsp70 +35//-12 S/X6	30) (empty, no data)
31)-190 hsp70 wt mp18	32)-190 hsp70 *TATA mp18	33)-190 hsp70 -30 mutation mp18	34)-190 hsp70 -2 mutation mp18	35)-190 hsp70 -30,-2 mutation mp18	36)-190 hsp70 wt mp19	37)-190 hsp70 *TATA mp19	38)-190 hsp70 -30 mutation mp19	39)-190 hsp70 -2 mutation mp19	40)-190 hsp70 -30,-2 mutation mp19
41) hsp70 wt -89 mp18	42)hsp70 *TATA -89 mp18	43)hsp70 wt -89 mp18	44)hsp70 *TATA -89 mp18	45)hsp70 wt -50 mp18	46)hsp70 *TATA -50 mp18	47)hsp70 wt -50 mp18	48)hsp70 *TATA -50 mp18	49)hsp70 -89 wt (reversed) mp19	50)hsp70 -89 *TATA (reversed) mp19
51)hsp70 S/X	52)hsp70 S/X-6	53)hsp70 term L	54)hsp70 term R	55)hsp70 XBS wt	56)hsp70 XBS *TATA	57) CaSpeR 1	58)-194// +84 Car20ZT.2	59) hsp70 *TATA -89 mp19	60) hsp70 *TATA -89 mp18
61)-30 in pUC XBS	62)-2 in pUC XBS	63)-30/ -2 in pUC XBS	64)H3 X-S Car20	65)H3 * X-S Car20	66)-50 mG Car20	67) *TATA Car20	68) p13wc	69) CCCAAA in Car20	70)Worst Inr in pUC XBS
71). -30/-2 in pUC XBS	72) Car20 ZT.2	73)-194 XBS wt	74)-194 *TATA XBS	75)-50mG in pUC XBS	76) CCCAAA in XBS	77) CCCAAA in Car20 ZT.2	78)Worst initiator in XBS	79)Worst initiator in Car20ZT.2	80)H3 initiator (in hsp70) in XBS
81)pGEM 7Zf(-)	82) pGEM 3Zf(+	83) pUC13 HSE8	84)pUC13 HSE8	85)pUC13 HSE8	86)T7 +61 clone A	87)T7 +61 clone B	88)T7 +61 clone C	89) pTZ19R	90)pGEM 3zf (+)
91)+42 pGEM 3zf	92)p3HB	93) (empty, no data)	94)p3HB	95)p3HB	96) (empty, no data)	97) (empty, no data)	98) (empty, no data)	99) -194*TA mp18	100) -194wt mp18

Description of contents:

Row 1 (return to top)
1)	hsp70 -190 mp18, double-stranded M13 DNA, 100 ng/ul
2)	hsp70 -89 mp18, double-stranded M13 DNA, 100 ng/ul
3)	hsp70 -50 mp18, double-stranded M13 DNA, 100 ng/ul
4)	pXM1 from Lis, dry tube
5)	pHT1 from Lis, 500 ng/ul
6)	pXM40 from Lis, multimer clone of hsp70 HSE 1 and 2, insert is quite heterogeneous in length
7)	pXM40 from Lis, multimer clone of hsp70 HSE 1 and 2, heterogeneity is unknown.
8)	hsp70 -190, pUC13 TATA -190 pUC13, same as slot 12 (also called hsp70 -190/TATA/+89 pUC13)
9)	-190 pUC13 -30/-2, same as slot 15, (also called hsp70 -190/-30,-2/+89 pUC13)
10)	hsp70 -190/-30/+89 pUC13

Row 2 (return to top)
11)	hsp70 -190//+89 pUC13
12)	hsp70 -190/*TATA/+89 pUC13
13)	hsp70 -190/-2/+89 pUC13
14)	hsp70 -190/-30/+89 pUC13
15)	hsp70 -190/-30,-2/+89 pUC13
16)	hsp70 -190 with dU for mutagenesis, single-stranded in mp18
17)	hsp70 -89 with dU for mutagenesis, single-stranded in mp18
18)	hsp70 -50 with dU for mutagenesis, single-stranded in mp18
19)	hsp70 S/X-1 extends to -190 in the hsp26 S/X vector, 3 mg/ml
20)	hsp70 S/X-1 extends to -190 in the hsp26 S/X vector, 1 mg/ml

Row 3 (return to top)
21)	-190 hsp70 wt mp18, double-stranded DNA
22)	-190 hsp70 *TATA mp18, double-stranded DNA
23)	-190 hsp70 -30 mutation mp18, double-stranded
24)	-190 hsp70 -2 mutation mp18, double-stranded
25)	-190 hsp70 -30,-2 mutation mp18, double-stranded
26)	hsp70 +35//-12 S/X3, dry tube
27)	hsp70 +35//-12 S/X1, 2.1 mg/ml
28)	hsp70 +35//-12 S/X1, 1.8 mg/ml
29)	hsp70 +35//-12 S/X6, 800 ug/ml
30)	(empty, no data)

Row 4 (return to top)
31)	-190 hsp70 wt mp18, single-stranded DNA
32)	-190 hsp70 *TATA mp18, single-stranded DNA
33)	-190 hsp70 -30 mutation mp18, single-stranded
34)	-190 hsp70 -2 mutation mp18, single-stranded
35)	-190 hsp70 -30,-2 mutation mp18, single-stranded
36)	-190 hsp70 wt mp19, single-stranded DNA, see X-link A box.
37)	-190 hsp70 *TATA mp19, single-stranded DNA
38)	-190 hsp70 -30 mutation mp19, single-stranded
39)	-190 hsp70 -2 mutation mp19, single-stranded
40)	-190 hsp70 -30,-2 mutation mp19, single-stranded

Row 5 (return to top)
41)	hsp70 wt -89 mp18, double-stranded
42)	hsp70 *TATA -89 mp18, double-stranded
43)	hsp70 wt -89 mp18, single-stranded
44)	hsp70 *TATA -89 mp18, single-stranded
45)	hsp70 wt -50 mp18, double-stranded
46)	hsp70 *TATA -50 mp18, double-stranded
47)	hsp70 wt -50 mp18, single-stranded
48)	hsp70 *TATA -50 mp18, single-stranded
49)	hsp70 -89 wt (reversed) mp19, single-stranded, see slot 7, X-link A box
50)	hsp70 -89 *TATA (reversed) mp19, single-stranded

Row 6 (return to top)
51)	hsp70 S/X extends to Xba1 site at -250, cloned in pUC13, 3.2 mg/ml
52)	hsp70 S/X-6, 8.6 mg/ml
53)	hsp70 term L, miniprep, Nov 89
54)	hsp70 term R, 1.88 mg/ml, 11/14/89
55)	hsp70 XBS wt from Purnell, has an Xho1 site at -190
56)	hsp70 XBS *TATA from Purnell, has Xho 1 site at -190, counterpart to DNA in slot 55
57)	CaSpeR 1, 0.5 ug/ul from Susan Abmayr.
58)	-194//+84 Car20ZT.2, 1.6 ug/ul, from Jan Weber
59)	hsp70 *TATA -89 mp19, single stranded (large "4" on lid)
60)	hsp70 *TATA -89 mp18, single stranded (large "2" on lid)

Row 7 (return to top)
61)	-30 in pUC XBS
62)	-2 in pUC XBS
63)	-30/-2 in pUC XBS
64)	H3 X-S Car20 made by JAW
65)	H3 * X-S Car20 made by JAW
66)	-50 mG Car20 made by JAW
67)	*TATA Car20 made by JAW
68)	p13wc, another tube at 0.26ug/ul
69)	CCCAAA in Car20 made by LVM
70)	Worst Inr in pUC XBS made by LVM

Row 8 (return to top)
71)	-30/-2 in pUC XBS
72)	Car20 ZT.2 ~1ug/ul
73)	-194 XBS wt from Sypes
74)	-194 *TATA XBS from Sypes
75)	-50mG in pUCXBS from JAW
76)	CCCAAA in XBS from LVM
77)	CCCAAA in Car20ZT.2 from LVM
78)	Worst initiator in XBS from LVM
79)	Worst initiator in Car20ZT.2 from LVM
80)	H3 initiator (in hsp70) in XBS from LVM

Row 9 (return to top)
81)	pGEM 7Zf(-)
82)	pGEM 3Zf(+)
83)	pUC13 HSE8 Choudhary 68ng/ul
84)	pUC13 HSE8 Choudhary 45ng/ul
85)	pUC13 HSE8 Choudhary 60ng/ul
86)	T7 +61 clone A Choudhary 137 ng/ul: T7 promoter region was amplified and inserted at NruI in +61
87)	T7 +61 clone B Choudhary 100 ng/ul
88)	T7 +61 clone C Choudhary 130 ng/ul
89)	pTZ19R from Babitzky
90)	pGEM 3zf (+) prepared by Hongbing

Row 10 (return to top)
91)	+42 pGEM 3zf prepared by Hongbing, RI/SalI fragment from Pete's +42 clone inserted into pGEM
92)	p3HB prepared by Hongbing, CsCl banded, used to make antisense and sense probes of the hsp70 promoter
93)	(empty, no data)
94)	p3HB, 1:250
95)	p3HB, 1:5
96)	(empty, no data)
97)	(empty, no data)
98)	(empty, no data)
99)	-194*TA mp18, virtually dry
100)	-194wt mp18, dry tube

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