Storage box form

Box: Chris' box
Location: Freezer next to ennzyme freezer
1) pP25.7	2) Car20 ZT.2-89	3) Car20ZT.2	4) hsp70wt	5) worst init.	6) p13wc	7) p13wc	8) pP25.7	9)
10) pCaSpeR-4	11) 5'LacZ.1	12) 1	13) 22	14) 272	15)worst init. Car20	16)hsp70 XBS	17)DTATA XBS	18)hsp70 +25
19) WT(white vector)	20) DTATA (white vector)	21) Inr (white vector)	22) +25/Inr (white vector)	23) +14(#2) (white vector)	24) +14 (#4) (white vector)	25) +30-+35	26 +30-+35	27) +25/INR mutant in white vector
28) -194 wt mp18	29) -194 DTA mp18	30) -89 wt mp18	31) -89 DTA mp18	32) -89 wt mp19	33) -89 DTA mp19	34) -89 wt mp18	35) -89 DTA mp18	36) 89 wt mp19
37) -89 DTA mp19	38) 0.1 mM ATP	39) 10 mM UTP	40) ddA mix	41) WT	42) RNase	43) 10 mM CTP	44) 10 mM rNTP	45) 10 mM GTP
46) Prot. K	47) tRNA	48) -89Car20	49) 100 mM Spermine	50) NTPs 10 mM	51)	52)	53)	54)
55) TR-1	56) TR-1	57) III seq. primer	58) +25 insert	59) +clone C	60) +clone C	61)	62)	63)
64)	65)	66)	67)	68)	69)	70)	71)	72)
73) X-gal	74) X-gal	75) Amp	76) 6X loading dye	77) l ladder	78) 6X loading dye	79)	80)	81) EtBr

Description of contents:

Row 1 (return to top)
1)	pP25.7, 78 ng/ul
2)	Car20 ZT.2-89, 7.2 mg/ml
3)	Car20ZT.2, 648 ng/ul, plasmid prep by Chris
4)	hsp70wt, 7.1 ng/ul, 1:1000 diltion of stock
5)	worst init., 382 ng/ul, plasmid prep by Chris 10/97
6)	p13wc, 410 ng/ul from Abmayr lab
7)	p13wc, 260 ng/ul
8)	pP25.7, 50 ng/ul, Quiagen prep, dirty DNA
9)

Row 2 (return to top)
10)	pCaSpeR-4, 640 ng/ul
11)	5'LacZ.1, BamHi/EcoRI fragment, 500 ng/ul
12)	1, 32 ng/ul (not sure about concentration)
13)	22, 10 ng/ul (not sure about concentration)
14)	272, 10 ng/ul (not sure about concentration)
15)	worst init. Car20ZT.2, 630 ng/ul
16)	hsp70 wt XBS, 7.1 ug/ul
17)	DTATA XBS, 681 ug/ml
18)	hsp70 +25, prepared by Hokuto, 6/23/99

Row 3 (return to top)
19)	hsp70WT(white vector), 450 ng/ul, in TE, constructed by Chris using 3-way ligation, verified by sequencing, 8/98
20)	DTATA (white vector), 490 ng/ul, in TE, constructed by Chris using 3-way ligation, verified by sequencing, 5/98
21)	Worst Initiator (white vector), 480 ng/ul, in TE, constructed by Chris using 3-way ligation, verified by sequencing, 5/98
22)	+25/worst Inr (white vector), 270 ng/ul, prepared by Chris by substituting the promoter of hsp70WT (white vector with Lakshmi's +25/Inr double mutant, 6/99 **NEEDS TO BE SEQUENCED, although restriction digests look correct
23)	+14 (#2) (white vector), 400 mg/ul, constructed by Chris by PCR-amplifying +14 mutatnt using Kate's primer and III seq. primer, verified by sequencing, Spring 1999
24)	+14(#4) (white vector), 435 ng/ul, constructed by Chris by PCR-amplifying +14 mutatnt using Kate's primer and III seq. primer, verified by sequencing, Spring 1999
25)	+30-+35, Tom's DNA, 1 ml, contaminated (most likely w/ carbohydrates)
26)	+30-+35, approx. 100 ng/ul, dirty DNA
27)	+25/INR mutant in white vector (750 ng/ul). DSG cleaned up 200ul of the prep in slot 22 by sperimine precipitation. The amount of DNA recovered was greater than expected suggesting that the DNA in slot 22 might be more concentrated than indicated.

Row 4 (return to top)
28)	-194 wt mp19 RF, 400 ng/ul, from Dave, 7/7/97
29)	-194 DTA mp19 RF, 400 ng/ul, from Dave, 7/7/97
30)	-89 wt mp18, from Dave
31)	-89 DTA mp18, from Dave
32)	-89 wt mp19, from Dave
33)	-89 DTA mp19, from Dave
34)	-89 wt mp18, phage prep
35)	-89 DTA mp18, phage prep
36)	-89 wt mp19, phage prep

Row 5 (return to top)
37)	-89 DTA mp19, phage prep
38)	0.1 mM ATP
39)	10 mM UTP
40)	10 mM GTP
41)	10 mM CTP
42)	10 mM rNTP
43)	NTP's 100 mM
44)	ddA Mix
45)	RNase

Row 6 (return to top)
46)	Proteinase K, 10 ug/ul
47)	tRNA 10 ug/ul
48)	-89Car20, in Salmon, 62.9 pg
49)	100 mM Spremine
50)
51)
52)
53)
54)

Row 7 (return to top)
55)	TR-1, 0.1 mM, primer, D1917, 01081801, TRANS-1
56)	TR-1, 1 uM, used in sequencing at BioTech
57)	III seq. primer, 7.2 pmole/ul, used in PCR reaction (with Kate's primer) for PCR amplification
58)	+25 insert, 7/10/??, made by Kate?
59)	Kate's +25 mutant, 31 ng/ul, used in sub-cloning
60)	Kate's +25 mutant, 18 ng/ul
61)
62)
63)

Row 8 (return to top)
64)
65)
66)
67)
68)
69)
70)
71)
72)

Row 9 (return to top)
73)	X-gal
74)	X-gal
75)	Amp (H2O), 50 mg/ml, sterilized, 10/97
76)	6X loading dye
77)	lambda DNA ladder, cut with HindIII, 120 ng/ul, 7/97
78)	6X loading dye
79)
80)
81)	EtBr stock

(return to top)

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