Our transformants
Transformants for transactivation project. The following table compiles lines that are believed to be homozygous.  All of these lines have the white gene as the transposition marker.  Those inserts that are identified as being on the 2nd or 3rd chromosome have been subjected to the homozygosing protocol.  Those inserts on the X chromosome were homozygosed via single pair matings.

Stock name	Eye color	Comments
TRE 70W 1	red	3rd chromosome.  Verified by Southern.
TRE 70W 2	red	3rd chromosome.  Verified by Southern.
TRE 70W 3or	red	2nd chromosome.  Verified by Southern.
TRE 70W 3x	red	X chromosome.  Discard because of incorrect banding on Southern.
TRE 70W 4		Verified by Southern.
TRE 70W 5r	red	3rd chromosome.  Southern suggests two inserts.
TRE 70W 5o	orange	2nd chromosome lethal.  Balanced over SM6a (curly phenotype).  Verified by Southern.
G5 70W 1y	yellow	2nd chromosome.  Verified by Southern.
G5 70W 2	red	3rd chromosome.  The stock has a mix of dark red and orange females.  Males are a brilliant red.   Verified by  Southern.
G5 70W 3x	orange	X chromosome.  Southern should be rechecked.  Possible choice for dysgenesis.  Failed last evlauation on Southern.
G5 70W 4x	orange	X chromosome.  Southern should be rechecked.  Verified by Southern
G2 70W 1x	orange	X chromosome.  Southern remains to be done.  Failed verification on Southern
G2 70W 3x	red	X chromosome.  Verified by Southern.  Possible choice for dysgenesis.
G2 70W 4x	orange	X chromosome.  Verified by Southern, may have two inserts.
G2 70w 6	orange	2nd chromosome.  Failed verification on Southern.

The following table compiles additional lines that have not been subjected to the homozygosing procedure.  These lines are being maintained by inter se mating. As of 10/23/98, all of the stocks listed in the above table are also being maintained as inter se stocks.  Those lines listed in the above table are not listed in the table below.  Although some of these are designated with an X to indicate X-linkage, it would be prudent to evaluate this.
 

Stock name	Eye color	Comments
TRE 70w 4	red and orange	---
G2 70w 2y	---	---
G2 70w 5x	orange and yellow	---
G2 70w 6x	---	---

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Transformants for core promoter analysis.

Stock name	Eye color	Comments
WT A (aka A.1)	red orange	Chromosome unknown, probably not X-linked.  Homozygous lethal and/or infertile. CPRG (for female) - high. Moderate-to-dark X-gal staining of adult tissues (O/N).Verified by Southern.
WT D (aka D.1)	yellow orange	X chromosome. Homozygous. CPRG (for female)- moderate.  Moderate X-gal staining of adult tissues (O/N).Verified by Southern.
WT D A.1.1	yellow	potential new line -needs to be cut with RV/XhoI**.
WT D A.2.1	yellow	Chromosome unknown.  Verified by Southern.
WT E (aka E.1)	yellow orange	2nd chromosome. CPRG (for female)- moderate to low.  Weak-to-moderate X-gal staining in adult tissue (O/N). Verified by Southern.
WT E.2	orange	X chromosome.  Verified by Southern and distinct from WT D (Benini, fall 99). Homozygous
Inr A (aka A.1)	yellow orange	X chromosome. CPRG - moderate to low.  Homozygous. Moderate X-gal staining of adult tissue (O/N). Verified by Southern.
Inr A A.1.1	yellow	potential new line -needs to be cut with RV/XhoI**. Homozygous
Inr A D.1.1		From dysgenisis - requires verification
Inr A D.1.2		From dysgenisis - requires verification
Inr A F.3.1		From dysgenic cross, uncharacterized
Inr A F.3.2		From dysgenic cross, linked to SM6a (curly)
Inr B (aka B.1)	yellow orange	2nd chromosome. Homozygous. CPRG - moderate. Moderate X-gal staining of adult tissue (O/N). Verified by Southern.
Inr B A.1.1	light orange	potential new line  Verified as transformant by Southern, but probably contains the original Inr B mutation, needs to be cut with RV/XhoI**
Inr B A.1.2	male red-orange, female orange	potential new line Verified as transformant by Southern, may contain multiple inserts.  I am out-crossing to separate the inserts.
delta TATA C	male red, female orange	2nd chromosome. CPRG - low. Random weak staining with X-gal along digestive tract of adult tissue (O/N). Verified by Southern.
delta TA C.3	orange	From dysgenic cross, linked to SM6a (curly).  Verified by Southern.
delta TA C.4	orange	From dysgenic cross, linked to SM6a (curly). Needs to be cut with RV/XhoI**
70w (+25) A.1	light orange	Hokuto's transformant - heterozygous
70w (+25) A.2		Hokuto's transformant - heterozygous
70w (+25) A.4		Hokuto's transformant - homozygous
70w (+25) A.7		Hokuto's transformant - homozygous.  Unusually low CPRG activity.
70w (+25) B.1		Hokuto's transformant - homozygous.
70w (+25) B.2		Hokuto's transformant - homozygous.
70w (+25) B.3		Hokuto's transformant - homozygous.

RV/XhoI **  When cut with EcoRV alone, these lines produce the desired, common length band.  However, the variable length band is very high molecular weight.  This band appears as a smear (probably because larger fragments are more suceptible to breakage and shearing).  A double digest might help reduce the size of these variable length bands and provide conclusive evidence that these lines contain new, unique inserts.

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White gene transformants that have unusal variations on the hsp70 promoter.

Stock Name	eye color	Comments
GAGA A.1		Benjamin's construct with GAGA element downstream from start.
LacO.1		Benjamin's construct with Lac operator downstream from start.

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The following table compiles what remains of some of our old transformed lines.  These lines are transformed with derivatives of our rosy vector called Car20 ZT.2.  Some of the information is based on a table I found in my lab notebook dated 7/14/95.
 

Old transformants based on rosy vector. (Note, some of these are in a cn- background)

Stock name	Phenotype	Comments
-194a	ry+	3rd chromosome, made by J. Weber
-194c	ry+	X-linked, made by J. Weber
Numerous -194c derivatives available from dysgenesis	---	---
-89 ln6	ry+	made by DSG
-89 ln2	ry+	made by DSG
-50A	ry+	our plasmid transformed by Lu Quinn
-50B	ry+	our plasmid transformed by Lu Quinn
-50C	ry+	our plasmid transformed by Lu Quinn
-50E	ry+	our plasmid transformed by Lu Quinn
-50mG	ry+	probably multiple inserts
-50mG 3.3.2b	ry+	possibly single insert
-50mG 3.2.3b	ry+	possibly single insert
deltaTA A1	ry+	DSG deletion of TATA box, maybe homozygous lethal, not X-linked
deltaTA A3	ry+	DSG deletion of TATA box, maybe homozygous lethal, not X-linked
deltaTA A5	ry+	DSG deletion of TATA box, maybe homozygous lethal
deltaTA B1	ry+	DSG deletion of TATA box, not X-linked
deltaTA C1	ry+	DSG deletion of TATA box, not X-linked
CCCA K	ry+	CCCA substitution of hsp70 TATA
CCCA L	ry+	CCCA substitution of hsp70 TATA
CCCA P	ry+	CCCA substitution of hsp70 TATA, this one is on the 4th and somewhat silenced.
Numerous CCCA derivatives available from dysgenesis
H3	ry+	H3 promoter fused to beta-gal, made by J. Weber
H3-11	ry+CyO	derived from H3 by dysgenesis, A test cross with rosy 506 showed that the insert is on the CyO balancer chromosome (chromosome 2).
H3-12	ry+	derived from H3 by dysgenesis
H3-18	ry+	derived from H3 by dysgenesis
deltaH3 A1	ry+	H3 promoter truncated immediately upstream from TATA
D7-6v6	ry+	Short form of histone repeat with H1 fused to beta-gal,  prepared when I was in Lis lab
Z243	ry+	hsp70 promoter fusion with beta-gal from the Lis lab.  Not X-linked