ALLEN T. PHILLIPS
Academic Training:
Louisiana State University, Baton Rouge, La., B.S.
in Biochemistry, 1960
Louisiana State University, Baton Rouge, La., M.S.
in Biochemistry, 1961
Michigan State University, East Lansing, Mich.,
Ph.D. in Biochemistry, 1964
Employment Record:
1964-1966: Assistant Professor of Biochemistry, Louisiana
State University
1967-1971: Associate Professor of Biochemistry,
Pennsylvania State University
1971-2001: Professor of Biochemistry, Pennsylvania
State University
2001-present: Emeritus Professor of Biochemistry,
Pennsylvania State University
Professional Society Memberships:
American Association for the Advancement of Science
American Chemical Society, Division of Biological
Chemistry
American Society of Biochemistry and Molecular Biology
American Society for Microbiology
Notable Awards Received:
NSF Undergraduate Research Fellowship, 1959
National General Foods Corporation Graduate Fellowship,
1960-61
NIH Predoctoral Graduate Fellowship, 1962-64
ASBC Travel Awards to International Congresses of
Biochemistry in Tokyo (1967), Stockholm (1973),
and Hamburg
(1976)
USPHS Research Career Development Award, 1972-77
Boezi Outstanding Alumnus Award, Dept. of Biochemistry,
Michigan State University, 1984
Fellow, American Academy of Microbiology, 1993
Major Professional Activities:
Editorial Board, Journal of Bacteriology, 1971-75,
1986-91
Editor, Journal of Bacteriology, 1975-82
Editorial Board, Applied and Environmental Microbiology,
1984-1998
President, Allegheny Branch, American Society for
Microbiology, 1991
Councilor, Allegheny Branch, American Society for
Microbiology, 1998-2000
Councilor, Division K, American Society for Microbiology,
1992-94
Chairman, Committee on Divisions, American Society
for Microbiology, 1994-98
Member, NIH Microbial Physiology & Genetics
Study Section, 1986-89
NIH Reviewer Reserve, 1989-96
NSF Presidential Young Investigator Award Panel,
1983
Department of Energy Biological Research Panel,
1985
USDA Competitive Grants Program Panel, 1997
Howard Hughes Research Institute Graduate Fellowship
Award Panel, 1998-2000
External Reviewer For:
USDA Cooperative Research Program, 1980-1,
1988, 1993, 1997
Veterans Administration, 1981-2, 1985, 1988-9
Louisiana Board of Regents, 1994
Nebraska Research Foundation, 1998
Sabbatical Leave Appointments:
1974: Visiting Scientist, Laboratory of Biochemical
Pharmacology, NIH, Bethesda, MD,
with Dr. Herbert Tabor, Chief
1982: Visiting Professor, Department of Biological
Sciences, Purdue University, W. Lafayette, IN,
with Dr. H. Edwin Umbarger
1992: Visiting Professor, Department of Biological
Sciences, Stanford University, Stanford, CA,
with Dr. Charles Yanofsky
Major Activities at Penn State:
Associate Department Head, 1984 - 1987
Acting Department Head, 1986
Chairman, Biochemistry Program, 1982 -
1990
University Isotopes Committee, 1985 -
2001 (Chairman from 1989 to 2001)
Graduate Council Member, 1988-91
University Faculty Senate, 1996-2000
Chair, Senate Committee on Faculty Benefits,
1998-2000
Member, University Undergraduate Research
Advisory Board, 1990-96
President's Planning and Budget Advisory
Committee, 1991
University Promotion and Tenure Committee,
1993-94
University Immediate Tenure Committee,
1994-2000
Faculty Advisor to the PSU Biochemistry
Society, 1982-2001
Faculty Technical Advisor to the PSU Bioprocessing
Resource Center, 1993-1998
Advisor to the Protein and DNA Synthesis
Laboratory, Biotechnology Institute, 1994
Fermentation Scale-Up Workshop Lecture Participant, Biotechnology Program,
1988-2001
Teaching and Research Training Activities:
Graduate courses:
enzyme kinetics and
protein chemistry (Bioch 525)
advanced microbiology
(Micrb. 536)
special topics in amino
acid transport (Bioch 589)
Undergraduate majors courses:
freshman orientation
course (Bioch 5)
general biochemistry
(Bioch [BMB] 401 and 402)
general biochemistry,
honors section (Bioch 401H)
laboratory in general
biochemistry (Bioch 403)
undergraduate majors
professional goals course (Bioch. 50)
undergraduate seminar
(Bioch. 451, BMB 411)
Undergraduate non-majors courses:
introductory biochemistry
(Bioch 241 and 242; BMB 211)
laboratory in introductory
biochemistry (Bioch 243)
Scholars Program students supervised in
thesis research: 14
Graduate students trained: 13 M.S., 14
Ph.D., 7 post-doctoral
Research Publications and Books
Tsai, H.Y., Phillips, A.T. and Williams, V.R. (1960). Molecular weights of crystalline amyloses from certain rice varieties. J. Agr. Food Chem. 8:364-367.
Phillips, A.T. and Williams, V.R. (1961). An investigation of varietal differences in the iodine-binding capacities of crystalline rice amyloses. J. Food Sciences 26:573-577.
Phillips, A.T. and Wood, W.A. (1964). Basis for AMP activation of biodegradative threonine dehydratase. Biochem. Biophys. Res. Commun. 15:530-535.
Phillips, A.T. and Wood, W.A. (1965). Mechanism of action of 5'-adenylic acid-acid activated threonine dehydratase. J. Biol. Chem. 240:4703-4709.
Phillips, A.T., Whanger, P.D., Rabinowitz, K.W., Shada, J.D., and Wood, W.A. (1968). Mechanisms and control of biodegradative threonine dehydrase. In: Pyridoxal Catalysis, (E.E. Snell, ed.), Interscience, New York, pp. 549-568.
Aaslestadt, H.G., Bouis, P.J., Phillips, A.T., and Larson, A.D. (1968). Characterization of a decarboxylation-dependent transaminase. In: Pyridoxal Catalysis, (E.E. Snell, ed.), Interscience, New York, pp. 479-490.
Phillips, A.T. (1968). Mechanism of the inactivation of threonine dehydratase during the dehydration of serine. Biochim. Biophys. Acta 151:523-526.
Vanquickenborne, A. and Phillips, A.T. (1968). Purification and regulatory properties of ADP-activated threonine dehydratase. J. Biol. Chem. 243:1312-1319.
Colvin, H.C. and Phillips, A.T. (1968). Inhibition of electron transport enzymes and cholinesterases by Endrin. Bull. Environ. Contamin. Toxicol. 3:106-115.
Whanger, P.D., Phillips, A.T., Rabinowitz, K.W., Piperno, J.R., Shada, J.D., and Wood, W.A. (1968). Mechanism of action of 5'-adenylic acid-activated threonine dehydrase. II. Protomer-oligomer interconversions and related properties. J. Biol. Chem. 243:167-173.
Vanquickenborne, A., Vidra, J.D., and Phillips, A.T. (1969). Independent modification of the binding sites for adenosine diphosphate and L-threonine in threonine dehydratase. J. Biol. Chem. 244:4808-4814.
George, D.J. and Phillips, A.T. (1970). Identification of a-ketobutyrate as the prosthetic group of urocanase from Pseudomonas putida. J. Biol. Chem. 245:528-537.
Magee, S.C. and Phillips, A.T. (1971). Molecular properties of the multiple aspartate aminotransferases purified from rat brain. Biochemistry 10:3397-3405.
Phillips, A.T. and George, D.J. (1971). Urocanase of Pseudomonas putida. In: Methods in Enzymology, Vol. 17B, (H. Tabor and C. Tabor, eds.), Academic Press, New York, pp. 73-79.
Mallette, M.F., Clagett, C.O., Phillips, A.T., and McCarl, R.L. (1971). Introductory Biochemistry, Williams and Wilkins, Baltimore, pp. 1-811.
Phillips, A.T., Nuss, J.I., Moosic, J., and Foshay, C. (1972). Alternate pathway for isoleucine biosynthesis in Escherichia coli. J. Bacteriol. 101:714-719.
Lynch, M.C. and Phillips, A.T. (1972). Urocanase of Pseudomonas putida. Subunit structure and origin of enzyme-bound a-ketobutyrate. J. Biol. Chem. 247:7799-7805.
Simon, J.P., Schorr, J.M., and Phillips, A.T. (1974). Interactions of 5'-adenosine diphosphate and L-threonine with the quaternary structure of L-threonine dehydratase. I. Nature of the enzymic substructure. J. Biol. Chem. 249:1993-1999.
Cohn, M.S. and Phillips, A.T. (1974). Purification and characterization of a B6-independent threonine dehydratase from Pseudomonas putida. Biochemistry 13:1208-1214.
Drummond, R.J. and Phillips, A.T. (1974). L-Glutamic acid decarboxylase in non-neural tissues of the mouse. J. Neurochem. 23:1207-1213.
Daumy, G.O., Cohn, M.S., and Phillips, A.T. (1974). Involvement of threonine dehydratase in biosynthesis of the a-ketobutyrate prosthetic group of urocanase. J. Bacteriol. 120:1249-1255.
Phillips, A.T. (1974). Ligand-induced oligomerization and regulatory mechanism. In: Critical Reviews in Biochemistry, Vol. 2, (G. Fasman, ed.), CRC Press, Cleveland, pp. 343-378.
Cohn, M.S., Lynch, M.C., and Phillips, A.T. (1975). Catalytic and thermodynamic properties of the urocanase hydratase reaction. Biochim. Biophys. Acta 377:444-453.
Drummond, R.J. and Phillips, A.T. (1977). Intracellular amino acid content of neuronal, glial and non-neural cell cultures: the relationship to glutamic acid compartmentation. J. Neurochem. 29:101-108.
Egan, R.M. and Phillips, A.T. (1977). Presence of a tightly bound NAD+ in urocanase of Pseudomonas putida. J. Biol. Chem. 252:5701-5707.
Phillips, A.T., LaJohn, L., and Lewis, B. (1977). O-Methylhydroxylamine as a reagent for NAD+ modification in urocanase. Arch. Biochem. Biophys. 184:215-221.
Egan, R.M. and Phillips, A.T. (1977). Requirements for induction of the biodegradative threonine dehydratase in Escherichia coli. J. Bacteriol. 132:370-376.
Phillips, A.T. (1977). Differential labeling: a general technique for selective modification of binding sites. In: Methods in Enzymology, Vol. 46, (W.B. Jakoby and M. Wilchek, eds.), Academic Press, New York, pp. 59-69.
King, S. and Phillips, A.T. (1978). Aromatic aminotransferase activity of rat brain cytoplasmic and mitochondrial aspartate aminotransferases. J. Neurochem. 30:1399-1407.
Phillips, A.T., Egan, R.M., and Lewis, B. (1978). Control of biodegradative threonine dehydratase inducibility by cyclic AMP in energy-restricted Escherichia coli. J. Bacteriol. 135:828-840.
Pishak, M.R. and Phillips, A.T. (1979). A modified radioisotopic assay for measuring glutamine synthetase activity in tissue extracts. Anal. Biochem. 94:82-88.
Nash, T.L. and Phillips, A.T. (1979). Regulatory properties of histidase from Pseudomonas putida. Biochem. Biophys. Res. Commun. 90:1229-1236.
Pishak, M.R. and Phillips, A.T. (1980). Glucocorticosteroid stimulation of glutamine synthetase production in cultured rat glioma cells. J. Neurochem. 34:866-872.
Matherly, L.H. and Phillips, A.T. (1980). Substrate mediated inactivation of urocanase from Pseudomonas putida. Evidence for an essential sulfhydryl group. Biochemistry 19:5814-5818.
Egan, R.M., Matherly, L.H., and Phillips, A.T. (1981). Mechanism of urocanase as studied by deuterium isotope effects and labeling patterns. Biochemistry 20:132-137.
Phillips, A.T. and Mulfinger, L.M. (1981). Cyclic adenosine 3',5'-monophosphate levels in Pseudomonas putida and Pseudomonas aeruginosa during induction and carbon catabolite repression of histidase synthesis. J. Bacteriol. 145:1286-1292.
Matherly, L.H., DeBrosse, C.W., and Phillips, A.T. (1982). A covalent nicotinamide adenine dinucleotide intermediate in the urocanase reaction. Biochemistry 21:789-2794.
Matherly, L.H., Johnson, K.A., and Phillips, A.T. (1982). Transient-state kinetic analysis of urocanase. Biochemistry 21:2795-2798.
Phillips, A.T. (1982). Function of NAD at the active site of urocanase. In: Methods in Protein Sequence Analysis, (M. Elzinga, ed.), Humana Press, Clifton, New Jersey, pp. 577-578.
Matherly, L.H. and Phillips, A.T. (1983). Identification of the enol tautomer of imidazolone propionate as the urocanase reaction product. Arch. Biochem. Biophys. 220:314-317.
Consevage, M.W. and Phillips, A.T. (1985). Presence and quantity of dehydroalanine in histidine ammonia-lyase from Pseudomonas putida. Biochemistry 24:301-308.
Consevage, M.W., Porter, R.D., and Phillips, A.T. (1985). Cloning and expression in Escherichia coli of histidine utilization genes from Pseudomonas putida. J. Bacteriol. 162:138-146.
Phillips, A.T. (1986). Biosynthetic and catabolic features of amino acid metabolism in Pseudomonas. In: The Bacteria, Vol. 10, "Biology of Pseudomonas", (J.R. Sokatch, ed.), Academic Press, New York, pp. 385-437.
Hu, L., Mulfinger, L.M., and Phillips, A.T. (1987). Purification and properties of formylglutamate amidohydrolase from Pseudomonas putida. J. Bacteriol. 169:4696-4702.
Hu, L. and Phillips, A.T. (1988). Organization and multiple regulation of histidine utilization genes in Pseudomonas putida. J. Bacteriol. 170:4272-4279.
Tsai, D.S., Arteca, R.N., Bachman, J.M., and Phillips. A.T. (1988). Purification and characterization of 1-aminocyclopropane-1-carboxylate synthase from etiolated mung bean hypocotyls. Arch. Biochem. Biophys. 264:632-640 (1988).
Hu, L., Allison, S.L., and Phillips, A.T. (1989). Identification of multiple repressor recognition sites in the hut system of Pseudomonas putida. J. Bacteriol. 171:4189-4195.
Consevage, M.W. and Phillips, A.T. (1990). Sequence analysis of the hutH gene encoding histidine ammonia-lyase in Pseudomonas putida. J. Bacteriol. 172:2224-2229.
Allison, S.L. and Phillips, A.T. (1990). Nucleotide sequence of the gene encoding the repressor for the histidine utilization genes of Pseudomonas putida. J. Bacteriol. 172:5470-5476.
Tsai, D.S., Arteca, R.N., Arteca, J.M., and Phillips, A.T. (1991). Characterization of 1-aminocyclopropane-1-carboxylate synthase purified from mung bean hypocotyls. J. Plant Physiol. 137:301-306.
Schlagnhaufer, C.D., Arteca, R.N., and Phillips, A.T. (1991). Induction of anti-brassinosteroid antibodies. J. Plant Physiol. 138:404-410.
Botella, J.R., Schlagnhaufer, C.D., Arteca, R.N., and Phillips, A.T. (1992). Identification and characterization of three putative genes for 1-aminocyclopropane-1-carboxylate synthase from etiolated mung bean hypocotyl segments. Plant. Mol. Biol. 18:793-797.
Cocco, M.J., Kao, Y.H., Phillips, A.T., and Lecomte, J.T. (1992). Structural comparison of apomyoglobin and metaquomyoglobin: pH titration of histidines by NMR spectroscopy. Biochemistry 31:6481-6491.
Botella, J.R., Arteca, J.M., Schlagnhaufer, C.D., Arteca, R.N. and Phillips, A.T. (1992). Identification and characterization of a full-length cDNA encoding for an auxin-induced 1-aminocyclopropane-1-carboxylate synthase from etiolated mung bean hypocotyl segments and expression of its mRNA in response to indole-3-acetic acid. Plant Mol. Biol. 20:425-436.
Guo, L., Arteca, R.N., Phillips, A.T., and Liu, Y. (1992). Purification and characterization of 1-aminocyclopropane-1-carboxylate N-malonyltransferase from etiolated mung bean hypocotyls. Plant Physiol. 100:2041-2045.
Botella, J.R., Schlagnhaufer, C.D., Arteca, J.M., Arteca, R.N., and Phillips, A.T. (1993). Identification of two new members of the 1-aminocyclopropane-1-carboxylate synthase multigene family in mung bean. Gene 123:249-253.
Phillips, A.T. (1993). Enzymatic activity. In: Methods for General and Molecular Bacteriology, (P. Gerhardt, ed.), American Society for Microbiology, Washington, DC, pp. 556-587.
Hernandez, D. and Phillips, A.T. (1993). Purification and characterization of Pseudomonas putida histidine ammonia-lyase expressed in Escherichia coli. Protein Expression and Purification, 4:473-478.
Hernandez, D., Stroh, J.G., and Phillips, A.T. (1993). Identification of Ser-143 as the site of modification in the active site of histidine ammonia-lyase. Arch. Biochem. Biophys. 307:126-132.
Guo, L., Phillips, A.T., and Arteca, R.N. (1993). Amino acid N-malonyltransferases from mung beans: action on 1-aminocyclopropane-1-carboxylic acid and D-phenylalanine. J. Biol. Chem. 268:25389-25394.
Hernandez, D., Phillips, A.T., and Zon, J. (1994). 1-Amino-2-imidazol-4'-ylethylphosphonic acid is a potent reversible inhibitor of Pseudomonas putida histidine ammonia-lyase. Biochemistry and Molecular Biology International 32:189-194.
King, R.S., Sechrist, L.L., and Phillips, A.T. (1994). A revised map location for the histidine utilization genes in Pseudomonas putida. J. Basic Microbiol. 34:253-257.
Hernandez, D. and Phillips, A.T. (1994). Ser-143 is an essential active site residue in histidine ammonia-lyase of Pseudomonas putida. Biochem. Biophys. Res. Commun. 201:1433-1438.
Walters, F.S., Kulesza, C.A., Phillips, A.T. and English, L.H. (1994). A stable oligomer of Bacillus thuringiensis delta-endotoxin, CryIIIA. Insect Biochem. Molec. Biol. 24:963-968.
Phillips, A.T. (1995). Dialysis and Concentration. In: Current Protocols in Protein Science, (H. Ploegh, D. Speicher, eds.), Academic Press, Inc., New York.
Wilfinger, W.W., Baker, C.S., Kunze, E.L., Phillips, A.T. and Hammerstedt, R.H. (1996). Versatile fluid-mixing device for cell and tissue microgravity research applications. J. Spacecraft and Rockets 33:126-130.
Teo, B., Kidd, R.D., Mack, J., Tiwari, A., Hernandez,
D., Phillips, A.T., and Farber, G.K. (1998). Crystallization and preliminary
X-ray studies of Pseudomonas putida histidine ammonium-lyase. Acta
Cryst. D54:681-683.