TCA precipitating small amounts of protein (5/19/00)

The following method is based on observations I made while developing a way to do in vitro protein-DNA crosslinking.  Some of these crosslinking experiments involved minute amounts of protein, and a TCA precipitation was used to remove free radioactive nucleotides that were present after crosslinking and nuclease digestion.

Procedure:


In this procedure, I think the detergent is acting as a carrier.  Although I don't know the details, I recall seeing another procedure where deoxycholate was used as a carrier.