RF1 |
|
|
RF2 |
|
Component (final concentration) |
Amount for 150 ml |
|
Component (final concentration) |
Amount for 50 ml |
100 mM KCl (74.55 mw) |
1.12 g |
|
10 mM MOPS pH 6.8 (209.3 mw) |
0.105 g |
50 mM MnCl2-4H2O (197.91 mw) |
1.48 g |
|
10 mM KCl (74.55 mw) |
0.037 g |
30 mM potassium acetate (98.14 mw) |
0.44 g |
|
75 mM CaCl2-2H2O (147.02 mw) |
0.55 g |
10 mM CaCl2-2H2O (147.02 mw) |
0.22 g |
|
15 % glycerol |
7.5 ml |
15% glycerol |
22.5 ml |
|
Adjust to pH 6.8 with 1 M NaOH and filter sterilize as
with RF1. |
Adjust to pH 5.8 with 10% glacial acetic acid, and filter
sterilize through a pre-rinsed 0.22 micron membrane. |